Showing 1 - 5 of 5 Items
The Role of Protein Kinases ROG1 and SRF6 in the WAK Stress Response Pathway
Date: 2015-05-01
Creator: Jaepil E Yoon
Access: Open access
Data associated with Walter-McNeill et al. (2021) Oecologia https://doi.org/10.1007/s00442-021-05038-y
Date: 2021-01-01
Creator: Barry Logan
Access: Open access
The Role of the Golgi ELMO Proteins in Cell Adhesion in Arabidopsis thaliana
Date: 2021-01-01
Creator: Wesley James Hudson
Access: Open access
- Proper growth and development of plant cells is dependent upon successful cell adhesion between cells, and this is mostly mediated by pectin in the plant cell wall. Previously, the Kohorn Laboratory identified a non-enzymatic Golgi protein named ELMO1 as it is required for cell adhesion, likely acting as a scaffold for cell wall polymer synthesis. Plants with mutant ELMO1 demonstrate a weak defective cellular adhesion phenotype as well as reduced mannose content in the cell wall. ELMO1 has homologous proteins in at least 29 different vascular plants. These homologues have 2 possible deletions in their amino acid sequence, but protein modeling determined that these variations will not affect protein structure. There are 5 homologous ELMO1 proteins in Arabidopsis thaliana that have been aptly named ELMO2, ELMO3, ELMO4, ELMO5. elmo2-/-mutants revealed no mutant adhesion phenotypes, while elmo1-/-elmo2-/-double mutants revealed strong defects in adhesion. Confocal microscopy of propidium iodide-stained seedlings confirmed the lack of a phenotype for elmo2-/-mutants and showed disorganized gapping cells for the elmo1-/-elmo2-/-mutant. Additionally, while elmo2-/-did not have any change to root or hypocotyl length, elmo1-/-elmo2-/- mutants were significantly shorter in both regards. Taken together, these data support that ELMO2 and ELMO1 are partially redundant.
Tracking photosynthetic seasonality at needle and forest scales in pines experiencing mild winters Access to this record is restricted to members of the Bowdoin community. Log in here to view.
- Restriction End Date: 2025-06-01
Date: 2022-01-01
Creator: Sara Elizabeth Nelson
Access: Access restricted to the Bowdoin Community
A histological investigation of Arceuthobium pusillum infections in Picea rubens and Picea glauca
Date: 2024-01-01
Creator: Sade K. McClean
Access: Open access
- Arceuthobium pusillum is a hemiparasite that infects select Picea species. The hosts of A. pusillum do not experience the same symptoms of infection. A. pusillum infections are more fatal to P. marinara, and P. glauca. P. rubens, on the other hand, can survive longer with sustained infection. This presents itself as a contemporary issue because P. glauca, one of the parasite’s most vulnerable hosts, was untethered from ecological competition when old growth forests were subjected to large scale anthropogenic disturbances. These disturbances allowed P. glauca to proliferate, with A. pusillum following. A deeper understanding of the host-species specific responses to A. pusillum infection can broaden general knowledge of parasitic growth and development while also potentially inspiring conservation techniques. This study took advantage of the intrinsic differences between host and parasite to visualize infections in P. rubens and P. glauca, highlighting differences in infection outcome. By illuminating lignin and callose within cross sections of infected P. rubens and P. glauca branches, it was revealed that P. rubens forms dense bands of cells around the cortical strands of infection. These bands form more frequently in P. rubens than in P. glauca and are of a significantly larger area in P. rubens than in P. glauca (t(8), p=0.003, p=0.005). The discovery of the exterior bands is novel and exciting, as the bands are possibly made of callose and potentially facilitate P. rubens survival against A. pusillum infection. The foundational discoveries and results of this study should inspire, and warrant, further analysis.