Showing 1 - 10 of 15 Items

• Embargo End Date: 2026-05-20

Date: 2021-01-01

Creator: Anneka Florence Williams

Access: Embargoed

Date: 2016-05-01

Creator: Benjamin M West

Access: Access restricted to the Bowdoin Community

Date: 2023-01-01

Creator: Nuoya (Laura) Yang

Access: Access restricted to the Bowdoin Community

Date: 2000-01-01

Creator: Nalini M. Nadkarni, Nathaniel T. Wheelwright

Access: Open access

The Monteverde Cloud Forest Reserve has captured the worldwide attention of biologists, conservationists, and ecologists and has been the setting for extensive investigation over the past 40 years. Roughly 40,000 ecotourists visit the Cloud Forest each year, and it is often considered the archetypal high-altitude rain forest. Featuring synthetic chapters and specific accounts written by more than 100 biologists and local residents, the 573-page book documents in a single volume everything known about the biological diversity of Monteverde, Costa Rica, and how to protect it. New short chapters which update and expand the research presented in the 2000 Oxford publication were written in 2014 and are now available.

• Restriction End Date: 2025-06-01

Date: 2022-01-01

Creator: Sara Elizabeth Nelson

Access: Access restricted to the Bowdoin Community

Date: 2024-01-01

Creator: Ayanna S Hatton

Access: Open access

When photons from sunlight are absorbed by plants, they can take paths of photosynthesis, fluorescence, or energy dissipation. Instruments to quantify fluorescence have expanded in scale to allow measurements from satellites and flux towers using Solar-Induced Chlorophyll Fluorescence (SIF). Studies have found a positive correlation between SIF and gross primary productivity (GPP; representative of photosynthesis), suggesting SIF is a proxy for GPP. This conclusion encourages the use of SIF to inform decisions about carbon budgets and responding to climate change. Studies of fluorescence on the single-leaf scale have revealed that SIF measurements do not account for all variables nor is there an understanding of the impact of environmental factors, such as drought, on these measurements. In this project, tall fescue turfgrass was placed in one of four differing drought severities for 19 days. Leaf-level measurements of photosynthesis and pulse-amplitude modulated fluorescence were made, demonstrating stomatal closure and inhibition of photosynthesis. This physiological change caused greater photon allocation to energy dissipation. Changes in greenness and the utilization of photoprotective mechanisms such as senescence and anthocyanin accumulation were observed. This study has provided an understanding of the temporal, physiological, and visible impacts of drought on turfgrass to inform interpretations of SIF in future experiments. Caution is crucial in utilizing SIF as a proxy for GPP before further research into the impact of drought on SIF is completed.

Date: 2019-05-01

Creator: Natasha Ann Belsky

Access: Access restricted to the Bowdoin Community

Date: 2014-05-01

Creator: Divya Hoon

Access: Access restricted to the Bowdoin Community

Date: 2017-01-01

Creator: Jack Ryan Mitchell

Access: Open access

Wall associated kinases (WAKs) are cell membrane bound receptor kinases that bind pectin and pectin fragments (OGs).The binding of WAKs to pectin sends a growth signal required for cell elongation and plant development. WAKs bind OGs with higher affinity than native pectin and instead activate a stress response. Glycine rich proteins (GRPs) are secreted cell wall proteins of unknown function. Seven GRPs with 65% sequence similarity are coded on a 90kb locus of Arabidopsis chromosome 2. GRP3 and WAK1 have been shown to bind in vitro, but single null mutations have no discernible phenotype, suggesting that the GRPs are redundant. Low recombination frequency has made multiple mutations difficult to achieve, but in this thesis, CRISPR/Cas9 technology was used to induce deletions of the GRP locus. The promoters pYAO and pICU2 drove Cas9 expression in transformed Arabidopsis plants. The presence of a deletion and Cas9 were detected by PCR. While somatic mutations were induced, there was no inheritance of the GRP deletion, indicating that pYAO and pICU2 do not drive Cas9 to induce deletions in progenitor cells. LIK1 is a CERK1 interacting kinase implicated in mediating response to various microbe associated molecular patterns (MAMP) such as chitin, flagellin, and peptidoglycans. LIK1 exhibits a drastic increase in phosphorylation in response to OG treatment, making it a candidate for a co-receptor to WAK. T-DNA insertions to the 5’UTR of LIK1 were used to examine the effect of a lik1 mutation on the OG induced stress response. lik1/lik1 mutant seedlings were grown in the presence and absence of OGs, and RNA was isolated. qPCR was used on cDNA to examine FADLOX expression, a reporter for the transcriptional response to OGs. The lik1/lik1 mutant caused a reduction in the OG induced transcriptional response. However, increased LIK1 expression was associated with the T-DNA insertion indicating that LIK1 inhibits the WAK stress response pathway. Understanding the roles of GRP and LIK1 in moderating WAK mediated pathogenic response in Arabidopsis will enable a better understanding of plant resistance to pathogen invasion in the greater plant kingdom.

Date: 2021-01-01

Creator: Andrew Close Bolender

Access: Open access

Plant cell adhesion is mediated by the extracellular matrix (ECM) or cell wall and plays an important role in plant morphogenesis and development. The amount, modification, and cleavage of pectin in the cell wall are major contributors to the adhesive properties of the ECM. To gain a more complete picture of plant cell adhesion processes, Arabidopsis thaliana seedlings were previously mutagenized and screened for hypocotyl adhesion defects. Genomic sequencing of one plant exhibiting an adhesion defect, isolate 242, showed that two mutations, one in cellulose synthase (CesA1) and another in a sugar transporter, are candidates for the causative mutation. This thesis reports that CesA1 is necessary for proper plant cell adhesion, while the sugar transporter encoded at At4g32390 is not. Dark grown seedlings homozygous for mutations in CesA1 stain in ruthenium red, indicating atypical adhesion, while those homozygous for null mutations in At4g32390 do not. Previous study of another adhesion mutant revealed ELMO1, a Golgi protein necessary for plant cell adhesion, and four additional homologs ELMO2-5 in the A. thaliana genome. Two of these homologs, ELMO2 and ELMO3, fused to GFP, colocalized with mCherry-MEM1 markers in the Golgi, but not mCherry-NLM12 ER markers, indicating that ELMO2 and ELMO3 are also Golgi proteins.