Showing 1 - 9 of 9 Items
Evaluation of design parameters for monosaccharide probes used in the metabolic labeling of bacterial glycans Access to this record is restricted to members of the Bowdoin community. Log in here to view.
Date: 2024-01-01
Creator: Sophia Elisabeth Nigrovic
Access: Access restricted to the Bowdoin Community
A semester-long project-oriented biochemistry laboratory based on Helicobacter pylori urease
Date: 2015-09-01
Creator: Kate R. Farnham, Danielle H. Dube
Access: Open access
- Here we present the development of a 13 week project-oriented biochemistry laboratory designed to introduce students to foundational biochemical techniques and then enable students to perform original research projects once they have mastered these techniques. In particular, we describe a semester-long laboratory that focuses on a biomedically relevant enzyme-Helicobacter pylori (Hp) urease-the activity of which is absolutely required for the gastric pathogen Hp to colonize the human stomach. Over the course of the semester, students undertake a biochemical purification of Hp urease, assess the success of their purification, and investigate the activity of their purified enzyme. In the final weeks of the semester, students design and implement their own experiments to study Hp urease. This laboratory provides students with an understanding of the importance of biochemistry in human health while empowering them to engage in an active area of research.
Identification and characterization of genes involved in Helicobacter pylori lipopolysaccharide and glycoprotein biosynthesis Access to this record is restricted to members of the Bowdoin community. Log in here to view.
Date: 2021-01-01
Creator: Andrew James Mulholland
Access: Access restricted to the Bowdoin Community
Investigating the Role of Helicobacter pylori Glycan Biosynthesis in Modulating Host Immune Cell Recognition Access to this record is restricted to members of the Bowdoin community. Log in here to view.
Date: 2023-01-01
Creator: Katharine Barrett
Access: Access restricted to the Bowdoin Community
Investigating the effect of Fuc-O-NAP on the glycosylation of Helicobacter pylori Access to this record is restricted to members of the Bowdoin community. Log in here to view.
Date: 2024-01-01
Creator: Panhasith Ung
Access: Access restricted to the Bowdoin Community
Recruiting the Host's Immune System to Target Helicobacter pylori's Surface Glycans
Date: 2013-04-01
Creator: Pornchai Kaewsapsak, Onyinyechi Esonu, Danielle H. Dube
Access: Open access
- Due to the increased prevalence of bacterial strains that are resistant to existing antibiotics, there is an urgent need for new antibacterial strategies. Bacterial glycans are an attractive target for new treatments, as they are frequently linked to pathogenesis and contain distinctive structures that are absent in humans. We set out to develop a novel targeting strategy based on surface glycans present on the gastric pathogen Helicobacter pylori (Hp). In this study, metabolic labeling of bacterial glycans with an azide-containing sugar allowed selective delivery of immune stimulants to azide-covered Hp. We established that Hp's surface glycans are labeled by treatment with the metabolic substrate peracetylated N-azidoacetylglucosamine (Ac4GlcNAz). By contrast, mammalian cells treated with Ac4GlcNAz exhibited no incorporation of the chemical label within extracellular glycans. We further demonstrated that the Staudinger ligation between azides and phosphines proceeds under acidic conditions with only a small loss of efficiency. We then targeted azide-covered Hp with phosphines conjugated to the immune stimulant 2,4-dinitrophenyl (DNP), a compound capable of directing a host immune response against these cells. Finally, we report that immune effector cells catalyze selective damage in vitro to DNP-covered Hp in the presence of anti-DNP antibodies. The technology reported herein represents a novel strategy to target Hp based on its glycans. © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
Metabolic inhibitors reshape Helicobacter pylori glycosylation Access to this record is restricted to members of the Bowdoin community. Log in here to view.
Date: 2020-01-01
Creator: Owen Templeton Tuck
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Identification of genes involved in Helicobacter pylori glycolipid and glycoprotein biosynthesis Access to this record is restricted to members of the Bowdoin community. Log in here to view.
Date: 2022-01-01
Creator: Adedunmola Praise Adewale
Access: Access restricted to the Bowdoin Community
Bacterial Coat of Armor: Probing how Glycan Biosynthesis in Helicobacter pylori Modulates Host Immune Recognition Access to this record is restricted to members of the Bowdoin community. Log in here to view.
Date: 2022-01-01
Creator: Francis Jacob Kassama
Access: Access restricted to the Bowdoin Community