Showing 1 - 7 of 7 Items
The EOL Enhancer Activates Eya Expression to Mediate Visual System Development in Drosophila melanogaster This record is embargoed.
- Embargo End Date: 2027-05-16
Date: 2024-01-01
Creator: Benjamin Sewell-Grossman
Access: Embargoed
The identification and visualization of candidate early embryonic patterning genes in Bradysia coprophila This record is embargoed.
- Embargo End Date: 2027-05-16
Date: 2024-01-01
Creator: Sarah Conant
Access: Embargoed
Characterization of Retinoic Acid Signaling During Tooth Morphogenesis and Evolution in Danio rerio This record is embargoed.
- Embargo End Date: 2028-05-17
Date: 2023-01-01
Creator: Lyn Stephanie Miranda Portillo
Access: Embargoed
Characterization of Spaetzle-Toll Ligand-Receptor Pairs in Gryllus bimaculatus Access to this record is restricted to members of the Bowdoin community. Log in here to view.
- Restriction End Date: 2028-06-01
Date: 2023-01-01
Creator: Tabarak Al Musawi
Access: Access restricted to the Bowdoin Community
Wnt Signaling is Dispensable to Formation of the First Tooth in D. Rerio This record is embargoed.
- Embargo End Date: 2025-05-14
Date: 2020-01-01
Creator: Zachary C. LeBlanc
Access: Embargoed
Examination of tooth-specific cis-regulation of the dlx2b gene during zebrafish development This record is embargoed.
- Embargo End Date: 2025-05-14
Date: 2020-01-01
Creator: Yujin Moon
Access: Embargoed
Injecting gryllus bimaculatus eggs
Date: 2019-08-01
Creator: Samantha K. Barry, Taro Nakamura, Yuji Matsuoka, Christoph Straub, Hadley W., Horch, Cassandra G. Extavour
Access: Open access
- Altering gene function in a developing organism is central to different kinds of experiments. While tremendously powerful genetic tools have been developed in traditional model systems, it is difficult to manipulate genes or messenger RNA (mRNA) in most other organisms. At the same time, evolutionary and comparative approaches rely on an exploration of gene function in many different species, necessitating the development and adaptation of techniques for manipulating expression outside currently genetically tractable species. This protocol describes a method for injecting reagents into cricket eggs to assay the effects of a given manipulation on embryonic or larval development. Instructions for how to collect and inject eggs with beveled needles are described. This relatively straightforward technique is flexible and potentially adaptable to other insects. One can gather and inject dozens of eggs in a single experiment, and survival rates for buffer-only injections improve with practice and can be as high as 80%. This technique will support several types of experimental approaches including injection of pharmacological agents, in vitro capped mRNA to express genes of interest, double-stranded RNA (dsRNA) to achieve RNA interference, use of clustered regularly interspaced short palindromic repeats (CRISPR) in concert with CRISPR-associated protein 9 (Cas9) reagents for genomic modification, and transposable elements to generate transient or stable transgenic lines.