Showing 1 - 10 of 10 Items
Investigating Candida albicans sensitivity to environmental stress in SR-like RNA binding
protein 1 C-terminal mutants lacking expression of Pin3 protein. Access to this record is restricted to members of the Bowdoin community. Log in here to view.
Date: 2025-01-01
Creator: Matthew Joseph Morales
Access: Access restricted to the Bowdoin Community
Investigating the effects of a glutamine-rich protein on the localization of a mutant RNA-binding protein and stress response in Candida albicans Access to this record is restricted to members of the Bowdoin community. Log in here to view.
- Restriction End Date: 2028-06-01
Date: 2023-01-01
Creator: Christoph Anders Tatgenhorst
Access: Access restricted to the Bowdoin Community
Localizing Potential Messenger RNA Transport Protein Ips1 in Candida albicans Access to this record is restricted to members of the Bowdoin community. Log in here to view.
- Restriction End Date: 2027-06-01
Date: 2022-01-01
Creator: Yi Peng Wang
Access: Access restricted to the Bowdoin Community
Impact of SR-Like RNA-Binding Protein (Slr1) Structure on Splicing in Candida albicans Access to this record is restricted to members of the Bowdoin community. Log in here to view.
- Restriction End Date: 2027-06-01
Date: 2022-01-01
Creator: Michael Christopher Dean
Access: Access restricted to the Bowdoin Community
Functionality of Candida albicans She3 in the mRNA transport of Saccharomyces cerevisiae Access to this record is restricted to members of the Bowdoin community. Log in here to view.
Date: 2021-01-01
Creator: Sheikh Omar Kunjo
Access: Access restricted to the Bowdoin Community
Testing conservation of an mRNA transport pathway in yeast Access to this record is restricted to members of the Bowdoin community. Log in here to view.
Date: 2021-01-01
Creator: Kyu Young "Kevin" Chi
Access: Access restricted to the Bowdoin Community
Role of SR-like RNA-binding protein 1 (Slr1) in hyphal tip localization of She3-transported mRNA in Candida albicans This record is embargoed.
- Embargo End Date: 2025-05-13
Date: 2020-01-01
Creator: Emma Beane
Access: Embargoed
Partner choice in spontaneous mitotic recombination in wild type and homologous recombination mutants of Candida albicans
Date: 2019-11-01
Creator: Alberto Bellido, Toni Ciudad, Belén Hermosa, Encarnación Andaluz, Anja, Forche, Germán Larriba
Access: Open access
- Candida albicans, the most common fungal pathogen, is a diploid with a genome that is rich in repeats and has high levels of heterozygosity. To study the role of different recombination pathways on direct-repeat recombination, we replaced either allele of the RAD52 gene (Chr6) with the URA-blaster cassette (hisG-URA3-hisG), measured rates of URA3 loss as resistance to 5-fluoroorotic acid (5FOAR) and used CHEF Southern hybridization and SNP-RFLP analysis to identify recombination mechanisms and their frequency in wildtype and recombination mutants. FOAR rates varied little across different strain backgrounds. In contrast, the type and frequency of mechanisms underlying direct repeat recombination varied greatly. For example, wildtype, rad59 and lig4 strains all displayed a bias for URA3 loss via pop-out/deletion vs. inter-homolog recombination and this bias was reduced in rad51 mutants. In addition, in rad51-derived 5FOAR strains direct repeat recombination was associated with ectopic translocation (5%), chromosome loss/truncation (14%) and inter-homolog recombination (6%). In the absence of RAD52, URA3 loss was mostly due to chromosome loss and truncation (80–90%), and the bias of retained allele frequency points to the presence of a recessive lethal allele on Chr6B. However, a few single-strand annealing (SSA)-like events were identified and these were independent of either Rad59 or Lig4. Finally, the specific sizes of Chr6 truncations suggest that the inserted URA-blaster could represent a fragile site.
Rapid phenotypic and genotypic diversification after exposure to the oral host niche in candida albicans
Date: 2018-07-01
Creator: Anja Forche, Gareth Cromie, Aleeza C. Gerstein, Norma V. Solis, Tippapha, Pisithkul, Waracharee Srifa, Eric Jeffery, Darren Abbey, Scott G. Filler, Aimée M. Dudley, Judith Berman
Access: Open access
- In vitro studies suggest that stress may generate random standing variation and that different cellular and ploidy states may evolve more rapidly under stress. Yet this idea has not been tested with pathogenic fungi growing within their host niche in vivo. Here, we analyzed the generation of both genotypic and phenotypic diversity during exposure of Candida albicans to the mouse oral cavity. Ploidy, aneuploidy, loss of heterozygosity (LOH), and recombination were determined using flow cytometry and double digest restriction site-associated DNA sequencing. Colony phenotypic changes in size and filamentous growth were evident without selection and were enriched among colonies selected for LOH of the GAL1 marker. Aneuploidy and LOH occurred on all chromosomes (Chrs), with aneuploidy more frequent for smaller Chrs and whole Chr LOH more frequent for larger Chrs. Large genome shifts in ploidy to haploidy often maintained one or more heterozygous disomic Chrs, consistent with random Chr missegregation events. Most isolates displayed several different types of genomic changes, suggesting that the oral environment rapidly generates diversity de novo. In sharp contrast, following in vitro propagation, isolates were not enriched for multiple LOH events, except in those that underwent haploidization and/or had high levels of Chr loss. The frequency of events was overall 100 times higher for C. albicans populations following in vivo passage compared with in vitro. These hyper-diverse in vivo isolates likely provide C. albicans with the ability to adapt rapidly to the diversity of stress environments it encounters inside the host.
Efficient and rapid identification of Candida albicans allelic status using SNP-RFLP
Date: 2009-11-01
Creator: Anja Forche, Musetta Steinbach, Judith Berman
Access: Open access
- Candida albicans is the most prevalent opportunistic fungal pathogen in the clinical setting, causing a wide spectrum of diseases ranging from superficial mucosal lesions to life-threatening deep-tissue infections. Recent studies provide strong evidence that C. albicans possesses an arsenal of genetic mechanisms promoting genome plasticity and that it uses these mechanisms under conditions of nutritional or antifungal drug stress. Two microarray-based methods, single nucleotide polymorphism (SNP) and comparative genome hybridization arrays, have been developed to study genome changes in C. albicans. However, array technologies can be relatively expensive and are not available to every laboratory. In addition, they often generate more data than needed to analyze specific genomic loci or regions. Here, we have developed a set of SNP-restriction fragment length polymorphism (RFLP) (or PCR-RFLP) markers, two per chromosome arm, for C. albicans. These markers can be used to rapidly and accurately detect large-scale changes in the C. albicans genome including loss of heterozygosity (LOH) at single loci, across chromosome arms or across whole chromosomes. Furthermore, skewed SNP-RFLP allelic ratios are indicative of trisomy at heterozygous loci. While less comprehensive than array-based approaches, we propose SNP-RFLP as an inexpensive, rapid, and reliable method to screen strains of interest for possible genome changes. © 2009 Federation of European Microbiological Societies.