Showing 1 - 8 of 8 Items
The Photocatalytic Degradation of Ibuprofen and Three Beta-Blockers using BiOCl: An Investigation into the Mechanisms Responsible for Photocatalytic Activity Access to this record is restricted to members of the Bowdoin community. Log in here to view.
- Restriction End Date: 2027-06-01
Date: 2022-01-01
Creator: Jeffrey Charles Price
Access: Access restricted to the Bowdoin Community
Identifying crustacean neuropeptides and precursor-related peptides by LC/MS: An investigation of strategies for extraction and orthogonal separations Access to this record is restricted to members of the Bowdoin community. Log in here to view.
- Restriction End Date: 2028-06-01
Date: 2023-01-01
Creator: Emily Grace Herndon
Access: Access restricted to the Bowdoin Community
Discovery and characterization of novel crustin family antimicrobial peptides (AMPs) in the American lobster, Homarus americanus, using transcriptomics and peptidomics Access to this record is restricted to members of the Bowdoin community. Log in here to view.
- Restriction End Date: 2027-06-01
Date: 2022-01-01
Creator: Emily Yuan-ann Pan
Access: Access restricted to the Bowdoin Community
The Determination of the Aqueous Oxidation Potentials of Aniline and Sixteen of its Derivatives via Ultrafast Cyclic Voltammetry to Model the Photocatalyzed Degradation of Organic Pollutants in Natural Bodies of Water Access to this record is restricted to members of the Bowdoin community. Log in here to view.
Date: 2014-05-01
Creator: Joshua V Pondick
Access: Access restricted to the Bowdoin Community
Structural and methodological factors influencing the sorption of alkylpyridiniums to aluminosilicates Access to this record is restricted to members of the Bowdoin community. Log in here to view.
Date: 2023-01-01
Creator: Seamus Frey
Access: Access restricted to the Bowdoin Community
Characterization of O-Linked Glycosylated Neuropeptides in the American Lobster (Homarus americanus): The Use of Peptide Labeling Following Beta Elimination
Date: 2020-01-01
Creator: Edward Myron Bull
Access: Open access
- Neuropeptides are a class of small peptides that govern various neurological functions, and the American lobster (Homarus americanus) provides a model system for their characterization. Neuropeptides are commonly post-translationally modified (PTM), and one common PTM is glycosylation. Past research in the Stemmler lab has found glycosylated neuropeptides in H. americanus; however, the extent and biological role of this modification has not been well characterized. This study was undertaken to determine the number of glycosylated peptides in the sinus glands of H. americanus and to develop an approach to tag the site of glycosylation using beta-elimination chemistry. LC-MS paired with high pH reverse phase fractionation was used to survey for glycosylated neuropeptides and beta elimination with an amine tag was used as an approach to characterize the site of glycosylation. Our results indicate that high pH fractionation is a useful approach to simplify complex mixtures of neuropeptides and improve glycopeptide detection. Efforts to use beta elimination and tagging to characterize glycosylated neuropeptides have been less successful. Beta elimination of full length peptides resulted in peptide degradation. An approach utilizing chymotrypsin to reduce peptide size coupled with beta elimination and labeling with 2-dimethylaminoethanethiol showed less evidence for degradation, and this approach yielded data isolating two potential serine residues for the site of glycosylation; however, the data was not sufficient to distinguish the two sites. Work to optimize reaction conditions using a glycopeptide standard showed that multiple isomeric products were formed during beta elimination. With the goal of optimizing reaction conditions, future work will further examine reaction kinetics to eventually apply the approach to the entire sinus gland
The Photocatalytic Degradation of 17α-ethynylestradiol (EE2) and Related Estrogens Access to this record is restricted to members of the Bowdoin community. Log in here to view.
- Restriction End Date: 2028-06-01
Date: 2023-01-01
Creator: Kevin Jairre Fleshman
Access: Access restricted to the Bowdoin Community
Reactions Responsible for Aging in Wood-Based Pyrolysis Oil: Synthesis and Characterization of a Coniferyl Alcohol Dimer
Date: 2021-01-01
Creator: Alejandro Garcia
Access: Open access
- The negative environmental impact and the diminishing supply of fossil fuels demand a renewable alternative. Pyrolysis oils produced from the decomposition of biomass, like wood, are a potential fuel substitute for energy production and a feedstock alternative for manufacturing value-added chemicals. The possibilities offered by pyrolysis oils are offset by oil instability. The oils contain reactive compounds, such as small aldehydes, conjugated aromatics, and acids that over time react and produce higher molecular mass products. This instability manifests as an increase in viscosity by a process referred to as aging. One chemical component, coniferyl alcohol, is proposed to react with formaldehyde under the acidic oil conditions to produce a dimer. In our lab, researchers have detected the coniferyl alcohol dimer in authentic oil samples and have simulated the reaction under conditions that removes the complexity of the pyrolysis oil matrix. This study focused on the synthesis, isolation, and characterization of the dimer structure by employing NMR analysis. GC/MS analysis of a successful synthesis of the dimer showed multiple dimers were produced, but there was one principal product. The NMR analysis of this dimer was used to elucidate the geometry, providing evidence that the product has E stereochemistry for the double bond and trans stereochemistry in the acetal ring. Confirmation of the principal structure provides support for the dimerization mechanism and will allow for future research to address instability of pyrolysis oils.