Showing 81 - 90 of 583 Items
Pyrokinin peptides’ effect on the stomatogastric nervous system in the American lobster, Homarus americanus
Date: 2014-08-01
Creator: Xuan Qu
Access: Open access
- Central pattern generators are networks of neurons that produce rhythmic and repetitiveoutputs. These outputs control behaviors such as walking, breathing and digestion. In the Americanlobster, central pattern generators control the behavior of muscles in its foregut, which allows thedigestion of a variety of food types. The stomatogastric ganglion (STG) is a bundle of about thirtyneurons in the foregut of American lobsters. It has been studied extensively since each one of theneurons in it is both identifiable and produces simple patterned outputs. The analysis of American lobster’s stomach behaviors and the neural mechanisms controlling them could provide general insights into how rhythmic motor patterns for locomotion are produced. A large number of the neurons in the STG are modulatory neurons that use neuromodulators for at least part of their synaptic receptions. These neuromodulators are released by neurons and cause long-lasting changes in the synaptic efficacies of the targets. At present, many types of neuropeptides have been identified within the crustacean stomatogastric nervous system. The pyrokinins are members of one peptide family, PBAN. PBAN peptides all share the common Cterminalpentapeptide FXPRL-amide, in which X can be S, T, G, N, or V. Previous studies, using immunohistochemistry, have found that there are pyrokinin peptides present in both the STG and the cardiac ganglion (CG) of American lobsters. My research tests five different kinds of pyrokinin peptides, including PevPK1 (DFAFSPRLamide) and PevPK2 (ADFAFNPRLamide) from the shrimp L.vannamei (Torfs et al., 2001; Ma et al., 2010), CabPK1 (TNFAFSPRLamide) and CabPK2(SGGFAFSPRLamide from the crab C.borealis (Saideman et al., 2007;Ma et al., 2009) and Conserved Sequence (FSPRLamide) from the lobster, H.americanus (Ma, et al, 2008). ConservedSequence, the only pyrokinin identified in the American lobster so far, is highly conserved among many other pyrokinin peptides. Therefore, it is believed to be just a fragment with the complete sequence yet to be identified. Thus, we predicted that it might produce a weaker effect on the STG. Previous studies on the pyrokinin peptides have shown that in crabs, CabPK1, CabPK2 and LeucoPK (identified in an insect), all had a virtually identical effect on the CG, suggesting that the differences among these pyrokinin peptides are not important and the receptors for these peptides are the same. However, research done by Bowdoin students in 2011-2012 showed that among PevPK1,PevPK2, CabPK1, CabPK2, and Conserved Sequence, all but Conserved Sequence (not yet tested) had strong effects on the STG. However, only PevPK2 had an effect on the CG. My goal for this summer research was to determine whether or not there are differences between the responses of the STG to the different peptides in order to further determine the cause for the differences between the responses of the CG and those of the STG. The results from the extracellular recordings from the identified neurons in my research have shown that none of the five kinds of pyrokinin peptides affect the pyloric rhythm, which controls the pumping and filtering of food through the pylorus in Americanlobsters. They all, however, excite the gastric mill rhythm, which controls the movements of the teeth that grind up the food before it is transferred into the pylorus. Moreover, there is no significant difference among the effects of these five kinds of pyrokinin peptides. Conserved Sequence, which was predicted to produce a relatively weaker effect, proved to produce virtually identical effect asfour other kinds of pyrokinin peptides. Future research will focus on studying the differences between the STG and CG to determine the cause of the varied responses between them. Final Report of research funded by the Doherty Coastal Studies Research Fellowship.
Traders and Troublemakers: Sovereignty in Southern Morocco at the End of the 19th Century
Date: 2020-01-01
Creator: Joseph Campbell Hilleary
Access: Open access
- This thesis explores changes in and challenges to Moroccan political authority in the region of the Sous during the late nineteenth century. It attempts to show how the phenomenon of British informal empire created a crisis over Moroccan sovereignty that caused the sultan to both materially and discursively change the way he wielded power in southern Morocco. It further connects these changes and the narrative contestation that accompanied them to the construction of the Bilad al-Siba/Bilad al-Makhzan dichotomy found in Western academic literature on Morocco starting in the colonial period. It begins with an examination of letters between Sultan Hassan I and local leaders in the Sous that show a shift toward a more bureaucratic form of governance in response to repeated openings of black-market ports by British trading companies. It then investigates the textual debate over the framing of Hassan I’s military expeditions to southern Morocco in the 1880s and 90s by drawing on a collection of European travel accounts, American consular reports, and a royal Moroccan history. Finally, it ties the illegal trade in the Sous to the broader theory of informal empire through a close examination of the Tourmaline Incident of 1897, using documents from the British Foreign Office as well as published accounts by crew members aboard the Tourmaline, itself.

Photosynthetic phenology of a boreal spruce forest observed at stand and needle scales This record is embargoed.
- Embargo End Date: 2025-05-19
Date: 2022-01-01
Creator: Jeremy A. Hoyne Grosvenor
Access: Embargoed

Finite Element Modeling of Piezoelectric Surface Wave Focusing Access to this record is restricted to members of the Bowdoin community. Log in here to view.
Date: 2022-01-01
Creator: Kieran Enzian
Access: Access restricted to the Bowdoin Community
A Problem Best Put Off Until Tomorrow
Date: 2023-01-01
Creator: Evan Albers
Access: Open access
- Effective Altruism has led a recent renaissance for utilitarian theory. However, it seems that despite its surge in popularity, Effective Altruism is still vulnerable to many of the critiques that plague utilitarianism. The most significant amongst these is the utility monster. I use Longtermsim, a mode of thinking that has evolved from Effective Altruism and prioritizes the far-future over the present in decision-making processes, as an example of how the unborn millions of the future might constitute a utility monster as a corporate mass. I investigate three main avenues of resolving the utility monster objection to Effective Altruism: reconsidering the use of expected value, adopting temporal discounting, and adopting average utilitarianism. I demonstrate that at best there are significant problems with these responses, and at worst, they completely fail to resolve the utility monster objection. I then conclude that if situations do exist in which the costs to the present do not intuitively justify the benefits to the far future, we must reject utilitarianism altogether.

Design, Fabrication, and Characterization of Unidirectional Interdigital Transducers Access to this record is restricted to members of the Bowdoin community. Log in here to view.
Date: 2022-01-01
Creator: Shane Anthony Smolenski
Access: Access restricted to the Bowdoin Community

The Regulatory Effect of High Dopamine on the Hyperpolarization-Activated Inward Current (Ih) and its Role in the Stability and Rhythmicity of Mammalian Locomotor Neural Networks Access to this record is restricted to members of the Bowdoin community. Log in here to view.
- Restriction End Date: 2025-06-01
Date: 2022-01-01
Creator: Abigail Raymond
Access: Access restricted to the Bowdoin Community
Exploiting Context in Linear Influence Games: Improved Algorithms for Model Selection and Performance Evaluation
Date: 2022-01-01
Creator: Daniel Little
Access: Open access
- In the recent past, extensive experimental works have been performed to predict joint voting outcomes in Congress based on a game-theoretic model of voting behavior known as Linear Influence Games. In this thesis, we improve the model selection and evaluation procedure of these past experiments. First, we implement two methods, Nested Cross-Validation with Tuning (Nested CVT) and Bootstrap Bias Corrected Cross-Validation (BBC-CV), to perform model selection and evaluation with less bias than previous methods. While Nested CVT is a commonly used method, it requires learning a large number of models; BBC-CV is a more recent method boasting less computational cost. Using Nested CVT and BBC-CV we perform not only model selection but also model evaluation, whereas the past work was focused on model selection alone. Second, previously models were hand picked based on performance measures gathered from CVT, but both Nested CVT and BBC-CV necessitate an automated model selection procedure. We implement such a procedure and compare its selections to what we otherwise would have hand picked. Additionally, we use sponsorship and cosponsorship data to improve the method for estimating unknown polarity values of bills. Previously, only subject code data was used. This estimation must be done when making voting outcome predictions for a new bill as well as measuring validation or testing errors. We compare and contrast several new methods for estimating unknown bill polarities.
Investigating the role of eyes absent in photoreceptor axon targeting in Drosophila melanogaster
Date: 2021-01-01
Creator: Bethany J. Thach
Access: Open access
- The eya gene is essential to development of the Drosophila visual system and eye-specific loss of function mutations in the gene commonly result in the missing eye phenotype. The eya2m35g mutation carries a deletion of exon 1B and adjacent regulatory sequences. Flies carrying the eya2m35g allele exhibit a photoreceptor axon phenotype that has not previously been associated with the eya gene. To determine a potential role for eya in photoreceptor axon targeting, I characterized various phenotypes of eya2m35g mutants and generated additional eya alleles consisting of smaller deletions within the eya2m35g mutation to locate the genetic source of axonal disruption. Using immunofluorescence staining to visualize Eya protein, I found a loss of eya expression in the optic lobe region of eya2m35g stage 9 embryos and third instar larvae. I also observed a loss of retinal basal glial (RBG) cells in the larval eye disc. Finally, I demonstrated that the disconnected axon phenotype is generated when a region of the intron immediately downstream of exon 1B is deleted. These findings suggest that a possible regulatory element for eya that is essential for photoreceptor axon targeting exists in this intronic region.
Characterizing the influence of Atlantic water intrusion on water mass formation and primary production in Kongsfjorden, Svalbard
Date: 2015-05-01
Creator: Courtney Michelle Payne
Access: Open access
- With warming global temperatures and changes to large-scale ocean circulation patterns, warm water intrusion into Arctic fjords is increasingly affecting fragile polar ecosystems. This study investigated how warm Atlantic water intrusion and the tidewater glacial melting it causes impacted water mass formation and primary productivity in Kongsfjorden, Svalbard. Data were collected over a 2-week period during the height of the melt season in August near the Kronebreen/Kongsvegen glacier complex, the most rapidly retreating glacier in Spitsbergen. Since 1998, intruding waters have warmed between 4 and 5.5˚C, which has prevented sea ice formation and changed the characteristics of fjord bottom waters. Increased glacial melting in the last decade has changed the characteristics of surface waters in the fjord. Modeled light fields suggest that suspended sediment in this glacial meltwater has reduced the euphotic zone close to the ice face, preventing high primary production in both the consistent and intermittent sediment-laden meltwater plumes. However, measurements collected close to terrestrially terminating glaciers indicate that extremely high primary production can occur in conditions of low turbidity. The results of this study support a three-part model of the effects of warm-water intrusion on water mass formation and primary production, where changes in sea ice coverage and tidewater glacial dynamics affect the optical light field. This model allows for spatial predictions for the most likely impacts of warm water intrusion on primary production in Spitsbergen, and could be extrapolated out to explore potential phytoplankton response in other regions susceptible to warm-water intrusion.