Showing 821 - 830 of 4695 Items
Date: 2005-09-02
Creator: Anne E. McBride
Jeffrey T. Cook
Elizabeth A. Stemmler
Kate L. Rutledge
Kelly A., McGrath
Jeffrey A. Rubens
Access: Open access
- Arginine methylation can affect both nucleocytoplasmic transport and protein-protein interactions of RNA-binding proteins. These effects are seen in cells that lack the yeast hnRNP methyltransferase (HMT1), raising the question of whether effects on specific proteins are direct or indirect. The presence of multiple arginines in individual methylated proteins also raises the question of whether overall methylation or methylation of a subset of arginines affects protein function. We have used the yeast mRNA-binding protein Npl3 to address these questions in vivo. Matrix-assisted laser desorption/ionization Fourier transform mass spectrometry was used to identify 17 methylated arginines in Npl3 purified from yeast: whereas 10 Arg-Gly-Gly (RGG) tripeptides were exclusively dimethylated, variable levels off methylation were found for 5 RGG and 2 RG motif arginines. We constructed a set of Npl3 proteins in which subsets of the RGG arginines were mutated to lysine. Expression of these mutant proteins as the sole form of Npl3 specifically affected growth of a strain that requires Hmtl. Although decreased growth generally correlated with increased numbers of Arg-to-Lys mutations, lysine substitutions in the N terminus of the RGG domain showed more severe effects. Npl3 with all 15 RGG arginines mutated to lysine exited the nucleus independent of Hmtl, indicating a direct effect of methylation on Npl3 transport. These mutations also resulted in a decreased, methylation-independent interaction of Npl3 with transcription elongation factor Tho2 and inhibited Npl3 self-association. These results support a model in which arginine methylation facilitates Npl3 export directly by weakening contacts with nuclear proteins. © 2005 by The American Society for Biochemistry and Molecular Biology, Inc.
Date: 2003-01-01
Creator: E. C. Zeeman
M. L. Zeeman
Access: Open access
- In this paper we exploit the linear, quadratic, monotone and geometric structures of competitive Lotka-Volterra systems of arbitrary dimension to give geometric, algebraic and computational hypotheses for ruling out nontrivial recurrence. We thus deduce the global dynamics of a system from its local dynamics. The geometric hypotheses rely on the introduction of a split Liapunov function. We show that if a system has a fixed point p ∈ int R+n and the carrying simplex of the system lies to one side of its tangent hyperplane at p, then there is no nontrivial recurrence, and the global dynamics are known. We translate the geometric hypotheses into algebraic hypotheses in terms of the definiteness of a certain quadratic function on the tangent hyperplane. Finally, we derive a computational algorithm for checking the algebraic hypotheses, and we compare this algorithm with the classical Volterra-Liapunov stability theorem for Lotka-Volterra systems.
Date: 1992-10-29
Creator: S. G. Naculich
C. P. Yuan
Access: Open access
- In a recent paper, Chivukula and Golden claimed that the electroweak symmetry-breaking sector could be hidden if there were many inelastic channels in the longitudinal WW scattering process. They presented a model in which the W's couple to pseudo-Goldstone bosons, which may be difficult to detect experimentally. Because of these inelastic channels, thw WW interactions do not become strong in the TeV region. We demonstrate that, despite the reduced WW elastic amplitudes in this model, the total event rate (∼ 5000 extra longitudinal W+W- pairs produced in one standard SSC year) does not decrease with an increasing number of inelastic channels, and is roughly the same as in a model with a broad high-energy resonance and no inelastic channels. © 1992.
Date: 1999-01-01
Creator: Michael L. Bender
Mark O. Battle
Access: Open access
Date: 2015-01-20
Creator: Allen Sockabasin
Access: Open access
Date: 2009-11-20
Creator: Kermit V Lipez
Access: Open access
Biographial Note
Kermit Victor Lipez was born August 18, 1941, in Philadelphia, Pennsylvania, to Abraham Hyman Lipez and Beatrice (Mayerson) Lipez. He was graduated from Haverford College in 1963 and took his law degree from Yale Law School in 1967. In 1990, he obtained a master of laws degree from the University of Virginia School of Law. He began his legal career in the Civil Rights Division of the U.S. Dept. of Justice (1967-1968) before serving as special assistant and legal counsel to Maine Governor Ken Curtis (1968-1971). From 1971 to 1972, he served as a legislative aide to Senator Edmund Muskie and then entered private practice as an attorney in Maine. From 1985 to 1994, he presided as a justice of the Maine Superior Court, and from 1994 to 1998 he sat on the Maine Supreme Court. Since 1998, he has served as a federal judge of the United States Court of Appeals for the First Circuit.
Summary
Interview includes discussion of: family and educational background; early civil rights work; 1970 Maine gubernatorial election recount; Maine 1974 gubernatorial campaign; playing tennis; George Mitchell’s personal attributes; First Circuit Court of Appeals; Ken Curtis; Edmund S. Muskie’s staff.
Date: 2009-06-20
Creator: Michael 'Mike' Aube
Access: Open access
Biographial Note
Michael “Mike” Aube was born on July 17, 1950, in Biddeford, Maine, and grew up in the Biddeford-Saco area in a Franco-American bilingual family. His mother worked as a clerk/cashier at a grocery store; his father was an electrical worker for a small electrical contractor in Saco, then began his own small electrical contracting business. Mike attended Boston College from 1968-1972, majoring in history and education. Although he became a certified teacher, he entered the realm of government and politics, working for George McGovern in 1972, and in 1973 becoming president of Maine Young Democrats. He served on Maine’s Democratic State Committee and worked for George Mitchell. Between 1975-1980, he went to Washington, DC, to work for the Senate Budget Committee (Muskie was chairman of the committee) as a researcher and was then promoted to director of special projects in Maine. He stayed in Washington until 1981 as Senator George Mitchell’s executive assistant. He has worked with the Economic Development Administration of the Department of Commerce, with jurisdiction over Maine, Connecticut, and Rhode Island. At the time of this interview he was Maine state director for USDA Rural Development.
Summary
Interview includes discussion of: Aube’s work for the Economic Development Administration, USDA Rural Development; mayor of Bangor; the 1972 McGovern campaign; Mitchell as Democratic National Committeeman; Aube’s involvement in the Maine Young Democrats and working for Mitchell during the 1974 gubernatorial race; the 1973 Maine Public Power referenda; an anecdote about Mitchell being a light sleeper; an anecdote about retrieving the discarded campaign literature to reuse; factors that contributed to Mitchell’s loss in 1974; visiting the Mitchell family; the 1976 Muskie reelection campaign; Mitchell’s appointment to the Senate; the transition to Mitchell in the Senate office; an anecdote about how initially no one knew who Senator Mitchell was; Senator Mitchell saying he needed to become majority leader so that he could control the schedule; staffing the Environment and Public Works Committee and working on economic and community development issues; working as Mitchell’s executive assistant; Aube’s decision to leave Washington, D.C.; Aube’s decision to become a Republican; how Mitchell helped Aube’s daughter with an application to the Disney Corporation; and an anecdote about campaigning at Bowdoin College in 1974 when Mitchell recited the starting line-up of the Red Sox and their batting averages to earn a vote.
Date: 2008-08-19
Creator: James 'Jim' W Case
Access: Open access
Biographial Note
James W. “Jim” Case was born on June 21, 1945, in Chicopee, Massachusetts. His father was a firefighter and his mother was a homemaker who raised seven children; Jim was the fourth of five boys. His family was Irish-German Catholic, and his parents were involved in local politics. He grew up in a blue-collar mill town with a good public educational system; he attended Clark University, where he majored in psychology. He was drafted in November 1968 at the height of the Vietnam War and served two years as an administrator in a physical therapy clinic at an Army hospital. Subsequently, he attended the University of Maine School of Law, became active in politics as co-chair of the local Kennedy campaign committee, and developed an interest in labor law. He also worked on George Mitchell’s gubernatorial campaign in 1974. After law school he went to Washington, DC to work in Senator Muskie’s office, first as assistant counsel to the Committee on Environment and Public Works, which involved economic development and environmental policy, and later as legislative director. He was Senator Mitchell’s first administrative assistant, a post he held until 1981. At the time of this interview he was a partner in the McTeague, Higbee, Case, Cohen, Whitney & Toker law firm.
Summary
Interview includes discussions of: 1972 Muskie presidential primary race; 1974 Maine gubernatorial race; factors that contributed to the results of the 1974 election; Mitchell on the campaign trail; Mitchell’s role in Muskie’s 1976 reelection campaign; how Mitchell came to be appointed U.S. attorney and later a federal judge; the transition in the Senate office when Muskie moved over to the State Department; Mitchell getting committee assignments; issues he worked on in his first year of office; Finance Committee deregulation of the banking industry; Case’s predicting that Mitchell would be Democratic leader; Senate prayer breakfasts; Case’s bringing David Johnson in to replace himself when he left in 1981; Mitchell’s accessibility to Mainers and commitment to Maine; and the mutual loyalty between Senator Mitchell and his staff.
Date: 2009-04-20
Creator: Thomas 'Tom' A Daschle
Access: Open access
Biographial Note
Thomas Andrew Daschle was born on December 9, 1947, in Aberdeen, South Dakota, to Elizabeth B. Meier and Sebastian C. Daschle. He attended South Dakota State University, being graduated with a degree in political science in 1969. After college he served as an intelligence officer in the U.S. Air Force. He started in politics as a staff member to South Dakota Senator James Abourezk. In 1978, Daschle was elected to the U.S. House of Representatives and served for four terms there. In 1986, he ran for the U.S. Senate and won, serving until he lost the seat in the fall 2004 elections; he succeeded George Mitchell as Senate majority leader in the Senate in 1995. He served as a key advisor to Barack Obama in his presidential campaign of 2008 and was nominated to be the secretary of health and human services; his nomination proved controversial, and he withdrew his name from consideration. He has been heavily involved in working for health care reform. At the time of this interview, he served as a policy advisor at the firm Alston & Bird and was a senior fellow at the Center for American Progress.
Summary
Interview includes discussion of: becoming acquainted with Mitchell; making the move to the Senate from the House; Mitchell’s role as chairman of the 1986 Democratic Senatorial Campaign Committee; the Senate class of 1986; the Democratic trend in the 1986 elections; working in the Senate compared to working in the House; the similarities between Daschle and Mitchell and the foundation of their friendship; Daschle’s role as co-chair of the Policy Committee; the Policy Committee’s functions and Mitchell’s role in expanding its responsibilities; Mitchell’s interest in baseball; Daschle’s campaign and election to majority leader; the procedure of the Senate leader election; Mitchell’s advice to Daschle as his successor as majority leader and his help in making the transition; Daschle’s surprise at Mitchell’s retirement decision; the 1994 elections; the check scandal that involved the House bank; Byrd’s decision to move from majority leader to chair of the Appropriations Committee; how Mitchell ought to be remembered; the Bipartisan Policy Center; thoughts on how Mitchell would have been as a presidential candidate; and Mitchell’s and Daschle’s mutual interest in hearing and telling funny stories.