Showing 621 - 630 of 722 Items
Exploring film language with a digital analysis tool: The case of kinolab
Date: 2021-01-01
Creator: Allison Cooper
Fernando Nascimento
David Francis
Access: Open access
- This article presents a case study of Kinolab, a digital platform for the analysis of narrative film language. It describes the need for a scholarly database of clips focusing on film language for cinema and media studies faculty and students, highlighting recent technological and legal advances that have created a favorable environment for this kind of digital humanities work. Discussion of the project is situated within the broader context of contemporary developments in moving image annotation and a discussion of the unique challenges posed by computationally-driven moving image analysis. The article also argues for a universally accepted data model for film language to facilitate the academic crowdsourcing of film clips and the sharing of research and resources across the Semantic Web.
Balancing Survival and Extinction in Nonautonomous Competitive Lotka-Volterra Systems
Date: 1995-06-01
Creator: F. Montes de Oca
M. L. Zeeman
Access: Open access
- We generalise and unify some recent results about extinction in nth-order nonautonomous competitive Lotka-Volterra systems. For each r ≤ n, we show that if the coefficients are continuous, bounded by strictly positive constants, and satisfy certain inequalities, then any solution with strictly positive initial values has the property that n - r of its components vanish, whilst the remaining r components asymptotically approach a canonical solution of an r-dimensional restricted system. In other words, r of the species being modeled survive whilst the remaining n - r are driven to extinction. © 1995 Academic Press, Inc.
Simplified insertion of transgenes onto balancer chromosomes via recombinase-mediated cassette exchange
Date: 2012-05-01
Creator: Florence F. Sun
Justine E. Johnson
Martin P. Zeidler
Jack R. Bateman
Access: Open access
- Balancer chromosomes are critical tools for Drosophila genetics. Many useful transgenes are inserted onto balancers using a random and inefficient process. Here we describe balancer chromosomes that can be directly targeted with transgenes of interest via recombinase-mediated cassette exchange (RMCE). ©2012 Sun et al.
“Fanny Buitrago: La magia de contar historias”. A Body of One’s Own: Conversations with Caribbean and Latina Writers
Date: 2007-01-01
Creator: Nadia V. Celis Salgado
Fanny Buitrago
Access: Open access
"whimsical contrasts": Love and marriage in The Minister's Wooing and Our Nig
Date: 2011-03-01
Creator: Tess Chakkalakal
Access: Open access
Non-transgenic genome modifications in a hemimetabolous insect using zinc-finger and TAL effector nucleases
Date: 2012-09-17
Creator: Takahito Watanabe
Hiroshi Ochiai
Tetsushi Sakuma
Hadley W. Horch
Naoya, Hamaguchi
Taro Nakamura
Tetsuya Bando
Hideyo Ohuchi
Takashi Yamamoto
Sumihare Noji
Taro Mito
Access: Open access
- Hemimetabolous, or incompletely metamorphosing, insects are phylogenetically relatively basal and comprise many pests. However, the absence of a sophisticated genetic model system, or targeted gene-manipulation system, has limited research on hemimetabolous species. Here we use zinc-finger nuclease and transcription activator-like effector nuclease technologies to produce genetic knockouts in the hemimetabolous insect Gryllus bimaculatus. Following the microinjection of mRNAs encoding zinc-finger nucleases or transcription activator-like effector nucleases into cricket embryos, targeting of a transgene or endogenous gene results in sequence-specific mutations. Up to 48% of founder animals transmit disrupted gene alleles after zinc-finger nucleases microinjection compared with 17% after microinjection of transcription activator-like effector nucleases. Heterozygous offspring is selected using mutation detection assays that use a Surveyor (Cel-I) nuclease, and subsequent sibling crosses create homozygous knockout crickets. This approach is independent from a mutant phenotype or the genetic tractability of the organism of interest and can potentially be applied to manage insect pests using a non-transgenic strategy. © 2012 Macmillan Publishers Limited. All rights reserved.
Tainted resurrection: metal pollution is linked with reduced hatching and high juvenile mortality inDaphniaegg banks
Date: 2015-05-01
Creator: Mary A. Rogalski
Access: Open access
Messenger RNA transport in the opportunistic fungal pathogen Candida albicans
Date: 2017-12-01
Creator: Anne E. McBride
Access: Open access
- Candida albicans, a common commensal fungus, can cause disease in immunocompromised hosts ranging from mild mucosal infections to severe bloodstream infections with high mortality rates. The ability of C. albicans cells to switch between a budding yeast form and an elongated hyphal form is linked to pathogenicity in animal models. Hyphal-specific proteins such as cell-surface adhesins and secreted hydrolases facilitate tissue invasion and host cell damage, but the specific mechanisms leading to asymmetric protein localization in hyphae remain poorly understood. In many eukaryotes, directional cytoplasmic transport of messenger RNAs that encode asymmetrically localized proteins allows efficient local translation at the site of protein function. Over the past two decades, detailed mechanisms for polarized mRNA transport have been elucidated in the budding yeast Saccharomyces cerevisiae and the filamentous fungus Ustilago maydis. This review highlights recent studies of RNA-binding proteins in C. albicans that have revealed intriguing similarities to and differences from known fungal mRNA transport systems. I also discuss outstanding questions that will need to be answered to reach an in-depth understanding of C. albicans mRNA transport mechanisms and the roles of asymmetric mRNA localization in polarized growth, hyphal function, and virulence of this opportunistic pathogen.
Narrative-inspired generation of ambient music
Date: 2017-01-01
Creator: Sarah Harmon
Access: Open access
- An author might read other written works to polish their own writing skill, just as a painter might analyze other paintings to hone their own craft. Yet, either might also visit the theatre, listen to a piece of music, or otherwise experience the world outside their particular discipline in search of creative insight. This paper explores one example of how a computational system might rely on what they have learned from analyzing another distinct form of expression to produce creative work. Specifically, the system presented here extracts semantic meaning from an input text and uses this knowledge to generate ambient music. An independent measures experiment was conducted to provide a preliminary assessment of the system and direct future work.
CrI3 revisited with a many-body ab initio theoretical approach
Date: 2021-06-01
Creator: Tom Ichibha
Allison L. Dzubak
Jaron T. Krogel
Valentino R. Cooper
Fernando A., Reboredo
Access: Open access
- CrI3 has recently been shown to exhibit low-dimensional, long-range magnetic ordering from few layers to single layers of CrI3. The properties of CrI3 bulk and few-layered systems are uniquely defined by a combination of short-range intralayer and long-range interlayer interactions, including strong correlations, exchange, and spin-orbit coupling. Unfortunately, both the long-range van der Waals interactions, which are driven by dynamic, many-body electronic correlations, and the competing strong intralayer correlations, present a formidable challenge for the local or semilocal mean-field approximations employed in workhorse electronic structure approaches like density-functional theory. In this paper we employ a sophisticated many-body approach that can simultaneously describe long- and short-range correlations. We establish that the fixed-node diffusion Monte Carlo (FNDMC) method reproduces the experimental interlayer separation distance of bulk CrI3 for the high-temperature monoclinic phase with a reliable prediction of the interlayer binding energy. We subsequently employed the FNDMC results to benchmark the accuracy of several density-functional theory exchange-correlation approximations.