Showing 2831 - 2840 of 5240 Items
Taming the giant within
Date: 2019-01-01
Creator: Jack R. Bateman
David J. Anderson
Access: Open access
The parasexual cycle in Candida albicans provides an alternative pathway to meiosis for the formation of recombinant strains
Date: 2008-05-01
Creator: Anja Forche
Kevin Alby
Dana Schaefer
Alexander D. Johnson
Judith, Berman
Richard J. Bennett
Access: Open access
- Candida albicans has an elaborate, yet efficient, mating system that promotes conjugation between diploid a and α strains. The product of mating is a tetraploid a/α cell that must undergo a reductional division to return to the diploid state. Despite the presence of several "meiosis-specific" genes in the C. albicans genome, a meiotic program has not been observed. Instead, tetraploid products of mating can be induced to undergo efficient, random chromosome loss, often producing strains that are diploid, or close to diploid, in ploidy. Using SNP and comparative genome hybridization arrays we have now analyzed the genotypes of products from the C. albicans parasexual cycle. We show that the parasexual cycle generates progeny strains with shuffled combinations of the eight C. albicans chromosomes. In addition, several isolates had undergone extensive genetic recombination between homologous chromosomes, including multiple gene conversion events. Progeny strains exhibited altered colony morphologies on laboratory media, demonstrating that the parasexual cycle generates phenotypic variants of C. albicans. In several fungi, including Saccharomyces cerevisiae and Schizosaccharomyces pombe, the conserved Spo11 protein is integral to meiotic recombination, where it is required for the formation of DNA double-strand breaks. We show that deletion of SPO11 prevented genetic recombination between homologous chromosomes during the C. albicans parasexual cycle. These findings suggest that at least one meiosis-specific gene has been re-programmed to mediate genetic recombination during the alternative parasexual life cycle of C. albicans. We discuss, in light of the long association of C. albicans with warm-blooded animals, the potential advantages of a parasexual cycle over a conventional sexual cycle. © 2008 Forche et al.
Suspense-optimal college football play-offs
Date: 2014-01-01
Creator: Jarrod Olson
Daniel F. Stone
Access: Open access
- U.S. college football’s traditional bowl system, and lack of a postseason play-off tournament, has been controversial for years. The conventional wisdom is that a play-off would be a more fair way to determine the national champion, and more fun for fans to watch. The colleges finally agreed to begin a play-off in the 2014-2015 season, but with just four teams, and speculation continues that more teams will be added soon. A subtle downside to adding play-off teams is that it reduces the significance of regular season games.We use the framework of Ely, Frankel, and Kamenica (in press) to directly estimate the utility fans would get from this significance, that is, utility from suspense, under a range of play-off scenarios. Our results consistently indicate that play-off expansion causes a loss in regular season suspense utility greater than the gain in the postseason, implying the traditional bowl system (two team play-off) is suspense-optimal. We analyze and discuss implications for TV viewership and other contexts.
Scattering equations and virtuous kinematic numerators and dual-trace functions
Date: 2014-01-01
Creator: Stephen G. Naculich
Access: Open access
- Inspired by recent developments on scattering equations, we present a constructive procedure for computing symmetric, amplitude-encoded, BCJ numerators for n-point gauge-theory amplitudes, thus satisfying the three virtues identified by Broedel and Carrasco. We also develop a constructive procedure for computing symmetric, amplitude-encoded dual-trace functions τ for n-point amplitudes. These can be used to obtain symmetric kinematic numerators that automatically satisfy color-kinematic duality. The S n symmetry of n-point gravity amplitudes formed from these symmetric dual-trace functions is completely manifest. Explicit expressions for four- and five-point amplitudes are presented. © 2014 The Author(s).
Statement by Denise Altvater on November 20, 2013
Date: 2013-11-20
Creator: Denise Altvater
Access: Open access
Statement by Margaret Semple collected by Rachel George on January 16, 2015
Date: 2015-01-16
Creator: Margaret Semple
Access: Open access
Statement by Michael Augustine collected by Rachel George on November 17, 2014
Date: 2014-11-17
Creator: Michael Augustine
Access: Open access
Large-scale chromosomal changes and associated fitness consequences in pathogenic fungi
Date: 2014-01-01
Creator: Anja Forche
Access: Open access
- Pathogenic fungi encounter many different host environments to which they must adapt rapidly to ensure growth and survival. They also must be able to cope with alterations in established niches during long-term persistence in the host. Many eukaryotic pathogens have evolved a highly plastic genome, and large-scale chromosomal changes including aneuploidy, and loss of heterozygosity (LOH) can arise under various in vitro and in vivo stresses. Both aneuploidy and LOH can arise quickly during a single cell cycle, and it is hypothesized that they provide a rapid, albeit imprecise, solution to adaptation to stress until better and more refined solutions can be acquired by the organism. While LOH, with the extreme case of haploidization in Candida albicans, can purge the genome from recessive lethal alleles and/or generate recombinant progeny with increased fitness, aneuploidy, in the absence or rarity of meiosis, can serve as a non-Mendelian mechanism for generating genomic variation. © Springer Science+Business Media 2014.
Glycans in pathogenic bacteria - potential for targeted covalent therapeutics and imaging agents
Date: 2014-04-08
Creator: Van N. Tra
Danielle H. Dube
Access: Open access
- A substantial obstacle to the existing treatment of bacterial diseases is the lack of specific probes that can be used to diagnose and treat pathogenic bacteria in a selective manner while leaving the microbiome largely intact. To tackle this problem, there is an urgent need to develop pathogen-specific therapeutics and diagnostics. Here, we describe recent evidence that indicates distinctive glycans found exclusively on pathogenic bacteria could form the basis of targeted therapeutic and diagnostic strategies. In particular, we highlight the use of metabolic oligosaccharide engineering to covalently deliver therapeutics and imaging agents to bacterial glycans. © 2014 The Partner Organisations.
islet reveals segmentation in the amphioxus hindbrain homolog
Date: 2000-04-01
Creator: William R. Jackman
James A. Langeland
Charles B. Kimmel
Access: Open access
- The vertebrate embryonic hindbrain is segmented into rhombomeres. Gene expression studies suggest that amphioxus, the closest invertebrate relative of vertebrates, has a hindbrain homolog. However, this region is not overtly segmented in amphioxus, raising the question of how hindbrain segmentation arose in chordate evolution. Vertebrate hindbrain segmentation includes the patterning of cranial motor neurons, which can be identified by their expression of the LIM-homeodomain transcription factor islet1. To learn if the amphioxus hindbrain homolog is cryptically segmented, we cloned an amphioxus gene closely related to islet1, which we named simply islet. We report that amphioxus islet expression includes a domain of segmentally arranged cells in the ventral hindbrain homolog. We hypothesize that these cells are developing motor neurons and reveal a form of hindbrain segmentation in amphioxus. Hence, vertebrate rhombomeres may derive from a cryptically segmented brain present in the amphioxus/vertebrate ancestor. Other islet expression domains provide evidence for amphioxus homologs of the pineal gland, adenohypophysis, and endocrine pancreas. Surprisingly, homologs of vertebrate islet1-expressing spinal motor neurons and Rohon-Beard sensory neurons appear to be absent. (C) 2000 Academic Press.