Showing 261 - 270 of 2670 Items
Date: 2008-03-01
Creator: Aimee M. Eldridge
Deborah S. Wuttke
Access: Open access
- The Saccharomyces cerevisiae protein Cdc13 tightly and specifically binds the conserved G-rich single-stranded overhang at telomeres and plays an essential role in telomere end-protection and length regulation. The 200 residue DNA-binding domain of Cdc13 (Cdc13-DBD) binds an 11mer single-stranded representative of the yeast telomeric sequence [Tel11, d(GTGTGGGTGTG)] with a 3 pM affinity and specificity for three bases (underlined) at the 5′ end. The structure of the Cdc13-DBD bound to Tel11 revealed a large, predominantly aromatic protein interface with several unusual features. The DNA adopts an irregular, extended structure, and the binding interface includes a long (∼30 amino acids) structured loop between strands β2-β3 (L2-3) of an OB-fold. To investigate the mechanism of ssDNA binding, we studied the free and bound states of Cdc13-DBD using NMR spectroscopy. Chemical shift changes indicate that the basic topology of the domain, including L2-3, is essentially intact in the free state. Changes in slow and intermediate time scale dynamics, however, occur in L2-3, while conformational changes distant from the DNA interface suggest an induced fit mechanism for binding in the 'hot spot' for binding affinity and specificity. These data point to an overall binding mechanism well adapted to the heterogeneous nature of yeast telomeres. © 2008 The Author(s).
Date: 1994-01-01
Creator: D. N. Brown
J. Fast
R. L. McIlwain
T. Miao
D. H., Miller
M. Modesitt
D. Payne
E. I. Shibata
I. P.J. Shipsey
P. N. Wang
M. Battle
J. Ernst
Y. Kwon
S. Roberts
E. H. Thorndike
C. H. Wang
J. Dominick
M. Lambrecht
S. Sanghera
V. Shelkov
T. Skwarnicki
R. Stroynowski
I. Volobouev
G. Wei
P. Zadorozhny
M. Artuso
M. Goldberg
D. He
N. Horwitz
R. Kennett
R. Mountain
Access: Open access
- We have measured the vector-pseudoscalar mass splitting M(Ds*+)-M(Ds+)=144.220.470.37 MeV significantly more precisely than the previous world average. We minimize the systematic errors by also measuring the vector-pseudoscalar mass difference M(D*0)-M(D0) using the radiative decay D*0D0, obtaining [M(Ds*+)-M(Ds+)]-[M(D*0)-M(D0)] =2.090.470.37 MeV. This is then combined with our previous high-precision measurement of M(D*0)-M(D0), which used the decay D*0D00. We also measure the mass difference M(Ds+)-M(D+)=99.50.60.3 MeV, using the + decay modes of the Ds+ and D+ mesons. © 1994 The American Physical Society.
Date: 1994-01-01
Creator: Y. Kubota
M. Lattery
J. K. Nelson
S. Patton
D., Perticone
R. Poling
V. Savinov
S. Schrenk
R. Wang
M. S. Alam
I. J. Kim
B. Nemati
J. J. O'Neill
H. Severini
C. R. Sun
M. M. Zoeller
G. Crawford
C. M. Daubenmier
R. Fulton
D. Fujino
K. K. Gan
K. Honscheid
H. Kagan
R. Kass
J. Lee
R. Malchow
F. Morrow
Y. Skovpen
M. Sung
C. White
F. Butler
Access: Open access
- Using the CLEO II detector, we have obtained evidence for a new meson decaying to D0K+. Its mass is 2573.2-1.6+1.7±0.8±0.5 MeV/c2 and its width is 16-4+5±3 MeV/c2. Although we do not establish its spin and parity, the new meson is consistent with predictions for an L=1, S=1, JP=2+ charmed strange state. © 1994 The American Physical Society.
Date: 2004-07-01
Creator: Vladimir Douhovnikoff
Adelaide M. Cheng
Richard S. Dodd
Access: Open access
- The ecology and evolutionary potential of coast redwood (Sequoia sempervirens) is significantly influenced by the important role clonal spread plays in its reproduction and site persistence. In nine second-growth stands, amplified fragment length polymorphisms (AFLPs) were used to identify redwood clonal architecture. Clones (multistem genets) dominated sites by representing an average of 70% of stems measured, ranging in size from two to 20 stems. As a result, a relatively small number of genets can monopolize a disproportionate amount of site resources, are more likely to persist over time, and have greater on-site genetic representation. Clones were not limited to fairy-ring structures, but consisted of a wide range of shapes including concentric rings, ring chains, disjunct, and linear structures. Between-ramet distances of up to 40 m were measured, indicating that clonal reproduction is not limited to basal stump resprouting. Clonal structure in second-growth stands was similar to earlier reports from old growth, emphasizing the importance of site persistence and long-term, gradual site development. Smaller ramet numbers per genet in old growth is probably due to local within-genet self thinning. Management and conservation of redwoods will benefit from a better understanding of the dynamics and structure of clonal spread in these forests.
Date: 2019-05-31
Creator: Maura Allen
Laurel Mast
Access: Open access
- Maura Allen (Class of 2014) and Laurel Mast (Class of 2014) describe the transition to Bowdoin and meeting each other on the rugby team. Mast talks about taking extra courses many semesters, participating in plays, and being an avid sports fan. Allen speaks about being a year-round athlete, a facilitator for V-Space, and living in Quinby House. The pair discuss their love for the state of Maine but acknowledge the unique challenges that come from being so far from their homes in Colorado and Oregon. Allen and Mast also reflect on the burgeoning discussions surrounding cultural appropriation and hookup culture on campus.
Date: 2019-06-01
Creator: Richard Lustig
Access: Open access
- Richard “Rich” Lustig (Class of 1974) shares how his childhood vacations in Maine led him to Bowdoin. He speaks about the serendipitous nature of making friends and the enjoyable times he spent playing music with friends. Reminiscing on a study-away experience at St. Andrews University in Scotland, which turned into an independent study on early music, Lustig speaks about how Bowdoin allowed him to pursue a wide and varied array of passions. He describes directing a play through Masque and Gown. He also describes how he ended up joining Alpha Delta Phi, despite a general distaste for fraternity life. Additionally, Lustig reflects on the drinking culture and the lack of socioeconomic and racial diversity at the College.
Date: 2019-06-01
Creator: Frank Skornia
Access: Open access
- Frank Skornia (Class of 2004) describes how he found his way to Bowdoin, including a Class of 1968 alumnus who mentored him. He discusses pre-Orientation trips and settling in to Hyde Hall. He reminisces about his deep involvement in the technical side of theater, including working for both the Theater department and Masque and Gown, and innovating ways of utilizing the new Wish Theater. Skornia speaks about taking advantage of the wide range of courses and the support of faculty advisors and mentors, and talks about his decision to spend a year abroad at the University of York. He discusses the increasing environmentalism and political tensions of the time, and the atmosphere and feelings on campus surrounding the September 11 Terrorist Attacks.
- This project provides Bowdoin alumni an opportunity to share stories from their time at the College via brief oral history interviews conducted by Special Collections & Archives staff. Interviewees are encouraged to recount stories of what brought them to the College, pre-orientation trip memories, campus life, study abroad, and the people that shaped their Bowdoin experience, from fellow students to faculty and staff.
Date: 2019-06-01
Creator: Michael Jeng
Access: Open access
- Michael Jeng (Class of 1989) describes living in Winthrop Hall and building lifelong friendships with his roommates. He speaks about challenging himself to take classes outside of his Biology and Philosophy majors. He discusses various on-campus jobs, like selling ads for the Orient, and extracurricular activities such as playing squash and tennis. Jeng remembers organizing a 10k run for Bowdoin students against apartheid in support of divestment from South Africa and attending pro-choice rallies in Washington, D.C. He reminisces on volunteering for a variety of organizations, mentioning the Big Brother Little Brother Program. Jeng also reflects on learning more about himself while at Bowdoin, including exploring his sexuality, leading a balanced life, and interacting with people from all walks of life.