Showing 1851 - 1860 of 2360 Items
The effects of nitric oxide on the modulation of the cardiac system of the American lobster, Homarus americanus, via a peptide (GYSDRNLRFamide)
Date: 2014-08-01
Creator: Sophie Janes
Access: Open access
- The central pattern generator (CPG) is a neural network that controls the rhythmic patterned outputs, which generate locomotion as needed. The lobster provides a good model to study CPGs because it has a relatively simple CPG. The lobster CPG, or cardiac ganglion accommodates for a range of activities and changes in the environment (Cooke, 2002).The small lobster CG is made up of nine neurons that control the neurogenic heart. The lobster CG is located on the inner dorsal wall of the heart and forms long neurites that branch onto the heart muscle. The CG, through an intrinsic mechanism, generates patterned and rhythmic bursts to the heart (Cooke 2002).The H. americanus CG sends information to the heart muscle to regulate the heart beat. The patterned bursts from the CG need to be adjusted in response to changing demands, for example, activity level or blood volume. Two general mechanisms, intrinsic feedback and extrinsic neuromodulation, have been identified to facilitate this adjustment. Through an intrinsic feedback mechanism, the muscle sends information back to the CG via a positive pathway and a negative pathway. In the positive pathway, stretch-sensitive dendrites of cardiac neurons increase the frequency of heart contractions when stretched (Cooke 2002). In the negative pathway, nitric oxide (NO), produced by the cardiac muscle, slows the frequency (Mahadevan et al. 2004). The interplay between the negative and positive feedback pathways regulates the output of the CG. An extrinsic mechanism has also been identified to regulate the CG output. Chemical neuromodulators that are released either locally or as hormones signal to the heart or CG to modulate ganglion activity. The intrinsic and extrinsic mechanisms affect the contraction amplitude and frequency of the heart.Within this simple invertebrate organism, a complex layering of control exists. Studies of the effects of various extrinsic modulators suggest that these modulators may alter how the feedback pathways operate. I examined what effect the neuromodulator GYSDRNLRFamide (GYS), a peptide found in the lobster nervous system, has on the balance between the positive and negative pathways (Ma et al., 2008). Recent experiments have demonstrated that when GYS was applied at high concentrations in the whole heart, the frequency decreased. This suggests that GYS may play a role in the intrinsic feedback pathways, and likely enhances the negative pathway.I looked at if nitric oxide altered the modulation of the heartbeat frequency when enhanced by the extrinsic modulator, GYS. Based on previous experiments, I hypothesized that GYS allows the NO, or negative pathway to predominate. In order to test my hypothesis, I examined the effects of GYS when I removed nitric oxide, which allows the negative pathway to exist. I compared the characteristics of the heartbeat when saline was run through the heart to when GYS was run through the heart. I also compared the characteristics of the heartbeat when the NO inhibitor, L-NA, was applied to when GYS was applied in the presence of L-NA. I finally compared the changes in frequency between these two comparisons. I found a significant difference between the change in frequency of the heart perfused with GYS in saline as opposed to perfused with GYS in L-NA. GYS had a greater negative effect without L-NA. These results demonstrate that NO is likely the cause of the observed decrease in frequency. Final Report of research funded by the Doherty Coastal Studies Research Fellowship.
Within the Atrium: A Context for Roman Daily Life
Date: 1997-01-01
Creator: Anna-Maria Cannatella
Access: Open access
- "This brochure accompanies an exhibition of the same name at the Bowdoin College Museum of Art, Brunswick, Maine, from April 3 through June 8, 1997."
Characterization of Dissolved Organic Matter in Local Marine and Terrestrial Waters
Date: 2014-08-01
Creator: Anna Bearman
Access: Open access
- There are numerous anthropogenic pollutants present in both marine and terrestrial waters.1 Though many of these chemicals do not absorb light and therefore cannot undergo photolyic degradation on their own, dissolved organic matter (DOM), found alongside pollutants in natural aquatic waters, can act as a catalyst in the attenuating process of contaminants. DOM is a complex mixture of organic compounds derived from decaying plants, animals and microorganisms. Since DOM can absorb light, it can transfer energy to contaminants, allowing them to break into smaller and often less hazardous molecules. The behavior of DOM is largely determined by its functional chemical components, and the character of DOM is constantly changing with the environment. For example, two International Humic Substances Society standards Pony Lake DOM from Antarctica and Suwannee River DOM from Georgia demonstrate very different compositions and characteristics2. More important than simply identifying varying functional groups in DOM these standards, however, may be understanding how our local water in the Androscoggin River and Gulf of Maine behaves and attenuates contaminants. The goal for this project was to first isolate DOM from the Androscoggin River and the Gulf of Maine. DOM was extracted using the Thurman and Malcolm procedure,3 beginning with collection and filtration of water from the Androscoggin River boat launch and Simpson’s Point. The water was then run through a chromatography column, through the method of absorption chromatography the dissolved organic matter sticks to the resin within the column. DOM was then eluted from the column, concentrated, and protonated with an ion exchange column. The resulting concentrated DOM solution was then freeze-dried to obtain the final powdered DOM fraction. Because the quantity of DOM isolated from the Gulf of Maine was too small for characterization, we determined that a new collection method using equipment suited for sampling larger volumes of water will be necessary for future DOM characterization. Instead we focused on collecting samples from the Androscoggin on a weekly basis. Following isolation, the Androscoggin River DOM was dissolved in Type I water to make 3mg/L DOM samples and then characterized through UV-Vis absorption and 3D fluorescence Excitation-Emission Matrix (EEM) spectroscopy techniques. The data was then processed using the parallel factor analysis (PARAFAC) method.4 PARAFAC deconvolutes the fluorescence spectra into the distinct fluorescent components present in the complex DOM mixture (Figure 1). This preliminary analysis indicates that Androscoggin River DOM is made up of at least six specific fluorophores. In the future, I will identify the types of molecules responsible for each component signature and attempt to ascertain the relative concentration of each photoactive constituent in the DOM samples. This information will have significant implications for the photochemistry of natural and anthropogenic chemicals in natural waters. Funded by the Henry L. Grace Doherty Coastal Studies Research Fellowship and James Stacy Coles Summer Research Fellowship in Chemistry.
Hands to Work and Hearts to God: The Shaker Tradition in Maine
Date: 1969-01-01
Creator: Theodore Elliot
Access: Open access
- "Catalogue [published] on the occasion of an exhibition [at Bowdoin] of Shaker art, furniture, and objects, mostly of Maine manufacture, now at Sabbathday Lake"--Foreword