Showing 1511 - 1520 of 2039 Items
Hydrophobic core but not amino-terminal charged residues are required for translocation of an integral thylakoid membrane protein in vivo
Date: 1996-10-07
Creator: Benoit Baillet
Bruce D. Kohorn
Access: Open access
- The integral membrane protein cytochrome f contains an amino-terminal signal sequence that is required for translocation into the thylakoid membrane. The signal sequence contains a hydrophobic core neighbored by an amino-terminal charged residue. Mutations that introduce charged amino acids into the hydrophobic core are inhibitory to cytochrome f translocation, and thus render cells non-photosynthetic. We have isolated both nuclear and chloroplast suppressors of these mutations by selecting for restoration of photosynthetic growth of Chlamydomonas. Here we describe the characterization of two chloroplast, second site suppressor mutations. Both suppressors remove the positively charged amino acid that borders the amino terminus of the hydrophobic core, and replace this arginine with either a cysteine or a leucine. The existence of these suppressors suggests that the hydrophobic core can be shifted in position within the signal sequence, and analysis of triple mutants in the signal confirms this hypothesis. Thus this signal that mediates translocation into the thylakoid membrane is characterized by a hydrophobic region whose exact amino acid content is not critical, and that need not be flanked on its amino terminus by a charged residue.
A cell wall-associated, receptor-like protein kinase
Date: 1996-09-05
Creator: Zheng Hui He
Masaaki Fujiki
Bruce D. Kohorn
Access: Open access
- Physical connections between higher plant cell walls and the plasma membrane have been identified visually, but the molecules involved in the contact are unknown. We describe here an Arabidopsis thaliana protein kinase, designated Wak1 for wall-associated kinase, whose predicted extracytoplasmic domain contains several epidermal growth factor repeats and identity with a viral movement protein. Wak1 fractionates with insoluble material when plant tissue is ground in a variety of buffers and detergents, suggesting a tight association with the plant extracellular matrix. Immunocytochemistry confirms that Wak1 is associated with the cell wall. Enzymatic digestion of the cell wall allows the release of Wak1 from the insoluble cell wall fraction, and protease experiments indicate that Wak1 likely has a cytoplasmic kinase domain, and the EGF containing domain is extracellular. Wak1 is found in all vegetative tissues of Arabidopsis, and has relatives in other angiosperms, but not Chlamydomonas. We suggest that Wak1 is a good candidate for a physical continuum between the cell wall and the cytoplasm, and since the kinase is cytoplasmic, it also has the potential to mediate signals to the cytoplasm from the cell wall.
An Arabidopsis serine/threonine kinase homologue with an epidermal growth factor repeat selected in yeast for its specificity for a thylakoid membrane protein
Date: 1992-01-01
Creator: Bruce D. Kohorn
Steven Lane
Tracy A. Smith
Access: Open access
- A number of molecules have recently been described that effect the correct transport and assembly of cytoplasmically synthesized proteins to cellular membranes. To identify proteins that bind or modify other proteins during the process of membrane translocation, we developed a yeast selection scheme that employs the yeast transcriptional activator GAL4. This selection facilitates the isolation of cDNAs that encode proteases and binding proteins for known target peptide sequences. We report the isolation of an Arabidopsis cDNA encoding a polypeptide that can interact with the amino terminus of a light-harvesting chlorophyll a/b-binding protein (LHCP), a cytoplasmically synthesized protein that is integral to the chloroplast thylakoid membrane. The cDNA was selected in yeast from an Arabidopsis expression library for its ability to inhibit a transcriptional activator GAL4-LHCP fusion protein, but not inhibit native GAL4 protein. The LHCP aminoterminal sequences included in the fusion protein are known to regulate LHCP biogenesis and function. The Arabidopsis cDNA encodes a 595-amino acid protein with at least two functional domains, one with similarity to the family of proteinserine/threonine kinases and another that contains an epidermal growth factor repeat. The identification of an EGF repeat in Arabidopsis indicates that the motif is conserved between the plant and animal kingdoms. Hybridization studies indicate that this gene is likely to be present in other genera of plants. Its mRNA is detected in green leaves but not in other plant tissues or in etiolated plants. The specificity in yeast and the expression pattern in plants together are suggestive of a role for this protein kinase in the assembly or regulation of LHCP.
Elliptic models and M-theory
Date: 2000-06-12
Creator: Isabel P. Ennes
Carlos Lozano
Stephen G. Naculich
Howard J. Schnitzer
Access: Open access
- We give a unified analysis of four-dimensional elliptic models with N = 2 supersymmetry and a simple gauge group, and their relation to M-theory. Explicit calculations of the Seiberg-Witten curves and the resulting one-instanton prepotential are presented. The remarkable regularities that emerge are emphasized. In addition, we calculate the prepotential in the Coulomb phase of the (asymptotically-free) Sp(2N) gauge theory with Nf fundamental hypermultiplets of arbitrary mass. © 2000 Elsevier Science B.V. All rights reserved.
Combinatorial properties of Thompson's group F
Date: 2004-07-01
Creator: Sean Cleary
Jennifer Taback
Access: Open access
- We study some combinatorial consequences of Blake Fordham's theorems on the word metric of Thompson's group F in the standard two generator presentation. We explore connections between the tree pair diagram representing an element w of F, its normal form in the infinite presentation, its word length, and minimal length representatives of it. We estimate word length in terms of the number and type of carets in the tree pair diagram and show sharpness of those estimates. In addition we explore some properties of the Cayley graph of F with respect to the two generator finite presentation. Namely, we exhibit the form of "dead end" elements in this Cayley graph, and show that it has no "deep pockets". Finally, we discuss a simple method for constructing minimal length representatives for strictly positive or negative words.
Functional and mutational analysis of the light-harvesting chlorophyll a/b protein of thylakoid membranes.
Date: 1986-01-01
Creator: B. D. Kohorn
E. Harel
P. R. Chitnis
J. P. Thornber
E. M., Tobin
Access: Open access
- The precursor for a Lemna light-harvesting chlorophyll a/b protein (pLHCP) has been synthesized in vitro from a single member of the nuclear LHCP multigene family. We report the sequence of this gene. When incubated with Lemna chloroplasts, the pLHCP is imported and processed into several polypeptides, and the mature form is assembled into the light-harvesting complex of photosystem II (LHC II). The accumulation of the processed LHCP is enhanced by the addition to the chloroplasts of a precursor and a co-factor for chlorophyll biosynthesis. Using a model for the arrangement of the mature polypeptide in the thylakoid membrane as a guide, we have created mutations that lie within the mature coding region. We have studied the processing, the integration into thylakoid membranes, and the assembly into light-harvesting complexes of six of these deletions. Four different mutant LHCPs are found as processed proteins in the thylakoid membrane, but only one appears to have an orientation in the membrane that is similar to that of the wild type. No mutant LHCP appears in LHC II. The other two mutant LHCPs cannot be detected within the chloroplasts. We conclude that stable complex formation is not required for the processing and insertion of altered LHCPs into the thylakoid membrane. We discuss the results in light of our model.
One-instanton test of a Seiberg-Witten curve from M-theory: The antisymmetric representation of SU(N)
Date: 1998-11-16
Creator: Stephen G. Naculich
Henric Rhedin
Howard J. Schnitzer
Access: Open access
- One-instanton predictions are obtained from the Seiberg-Witten curve derived from M-theory by Landsteiner and Lopez for the Coulomb branch of N = 2 supersymmetric SU(N) gauge theory with a matter hypermultiplet in the antisymmetric representation. Since this cubic curve describes a Riemann surface that is non-hyperelliptic, a systematic perturbation expansion about a hyperelliptic curve is developed, with a comparable expansion for the Seiberg-Witten differential. Calculation of the period integrals of the SW differential by the method of residues of D'Hoker, Krichever, and Phong enables us to compute the prepotential explicitly to one-instanton order. It is shown that the one-instanton predictions for SU(2), SU(3), and SU(4) agree with previously available results. For SU(N), N ≥ 5, our analysis provides explicit predictions of a curve derived from M-theory at the one-instanton level in field theory. © 1998 Elsevier Science B.V.
Evaluation of the free energy of two-dimensional Yang-Mills theory
Date: 1996-01-01
Creator: Michael Crescimanno
Stephen G. Naculich
Howard J. Schnitzer
Access: Open access
- The free energy in the weak-coupling phase of two-dimensional Yang-Mills theory on a sphere for [Formula presented] and [Formula presented] is evaluated in the [Formula presented] expansion using the techniques of Gross and Matytsin. Many features of Yang-Mills theory are universal among different gauge groups in the large [Formula presented] limit, but significant differences arise in subleading order in [Formula presented]. © 1996 The American Physical Society.
String calculation of QCD Wilson loops on arbitrary surfaces
Date: 1995-01-01
Creator: Stephen G. Naculich
Harold A. Riggs
Access: Open access
- Compact string expressions are found for nonintersecting Wilson loops in SU(N) Yang-Mills theory on any surface (orientable or nonorientable) as a weighted sum over covers of the surface. All terms from the coupled chiral sectors of the 1/N expansion of the Wilson loop expectation values are included. © 1995 The American Physical Society.
Social Learning Strategies: Bridge-Building between Fields
Date: 2018-07-01
Creator: Rachel L. Kendal
Neeltje J. Boogert
Luke Rendell
Kevin N. Laland
Mike, Webster
Patricia L. Jones
Access: Open access
- While social learning is widespread, indiscriminate copying of others is rarely beneficial. Theory suggests that individuals should be selective in what, when, and whom they copy, by following 'social learning strategies’ (SLSs). The SLS concept has stimulated extensive experimental work, integrated theory, and empirical findings, and created impetus to the social learning and cultural evolution fields. However, the SLS concept needs updating to accommodate recent findings that individuals switch between strategies flexibly, that multiple strategies are deployed simultaneously, and that there is no one-to-one correspondence between psychological heuristics deployed and resulting population-level patterns. The field would also benefit from the simultaneous study of mechanism and function. SLSs provide a useful vehicle for bridge-building between cognitive psychology, neuroscience, and evolutionary biology.