Showing 141 - 150 of 257 Items
Localizing Potential Messenger RNA Transport Protein Ips1 in Candida albicans Access to this record is restricted to members of the Bowdoin community. Log in here to view.
- Restriction End Date: 2027-06-01
Date: 2022-01-01
Creator: Yi Peng Wang
Access: Access restricted to the Bowdoin Community
Effects of Origin Environment and Temperature Acclimation on the Temperate Coral Astrangia poculata This record is embargoed.
- Embargo End Date: 2026-05-18
Date: 2023-01-01
Creator: Deva K Holliman
Access: Embargoed
Examining the Ability of Remote Sensing to Characterize Turfgrass Stress Physiology
Date: 2022-01-01
Creator: Benjamin Ross
Access: Open access
- Remote sensing of solar induced chlorophyll fluorescence (SIF) is a valuable tool in understanding the global carbon cycle. While SIF is highly correlated with photosynthesis at the ecosystem scale, the role that remote sensing of SIF can play at smaller scales is still unclear. The goal of my research was to investigate the ability of SIF to detect changes in pigmentation, photosynthesis, and energy partitioning at the grass canopy and leaf level in response to water stress and abscisic acid (ABA) hormone treatments. Both treatments immediately inhibited photosynthesis by limiting gas exchange through stomatal closure, but SIF declined gradually. Recovery of photosynthesis after alleviation of water stress was not reflected in remote measurements of SIF. I found that senescence in the tips of grasses had been driving changes in remote measurements, which affected remote measurements even when measured leaf-level gas exchange in the lower living tissue recovered. This heterogeneous senescence pattern contextualizes the disconnect between SIF and photosynthesis in stressed turfgrass.
Mutual benefits of inducible defenses to crab predators in the blue mussel Mytilus edulis in a multi-predator environment
Date: 2021-01-01
Creator: Sophia Walton
Access: Open access
- The blue mussel Mytilus edulis alters its phenotype in species-specific ways in response to either green crab (Carcinus maenus) or sea star (Asterias sp.) predation. Previous studies have shown that only sea stars induce changes in abductor muscle morphology, while green crabs generally alter the shape and thickness of shells. In the Western Gulf of Maine, Blue mussels collected from wave protected sites with abundant green crab predators were shown to have significantly thicker shells and larger adductor muscles than mussels collected from wave exposed sites with few green crab predators. The phenotypes of mussels originating from wave-protected and high green crab abundance sites increased the handling time by A. forbesi compared to sites with low wave exposure and high green crab abundance. These results contradict the paradigm that shell thickness trades off with abductor morphology, and I propose that a likely candidate for increased energy allocation to these traits is a decrease in reproductive allocation. My results further suggest that the escalating “arms race” between invasive green crabs and blue mussels in the Western Gulf of Maine is leading to changes in the phenotypic response of mussel populations in ways that are likely impacting sea star foraging dynamics.
In situ HCR in non-traditional arthropods
Date: 2024-01-25
Creator: Heather Bruce, Hadley Wilson Horch
Access: Open access
- Visualizing the expression of genes is a fundamental tool in molecular biology. Traditional colorimetric in situ hybridization using long RNA probes has been a staple for visualizing gene expression but has many drawbacks. In situ HCR v3.0, developed by Choi et. al. 2018, offers improvements over traditional in situs in nearly every aspect: probes can simply be ordered rather than painstakingly cloned and transcribed, which also makes them cost-effective; an HCR takes just three days to complete rather than five or more days; HCR is robust and works well for first-time users; and HCR probes can be multiplexed, allowing four to eight genes to be visualized in a single sample. HCR has been used successfully in many arthropods, including insects (Drosophila, Tribolium), crustaceans (Parhyale, Daphnia, Artemia), and chelicerates (Limulus horseshoe crab, Acanthoscurria tarantula). In this demo, you will learn how to design and order HCR probes as well as best practices for experimental design.
Distinct or shared actions of peptide family isoforms: I. Peptidespecific actions of pyrokinins in the lobster cardiac neuromuscular system
Date: 2015-09-01
Creator: Patsy S. Dickinson, Anirudh Sreekrishnan, Molly A. Kwiatkowski, Andrew E. Christie
Access: Open access
- Although the crustacean heart is modulated by a large number of peptides and amines, few of these molecules have been localized to the cardiac ganglion itself; most appear to reach the cardiac ganglion only by hormonal routes. Immunohistochemistry in the American lobster Homarus americanus indicates that pyrokinins are present not only in neuroendocrine organs ( pericardial organ and sinus gland), but also in the cardiac ganglion itself, where pyrokinin-positive terminals were found in the pacemaker cell region, as well as surrounding the motor neurons. Surprisingly, the single pyrokinin peptide identified from H. americanus, FSPRLamide, which consists solely of the conserved FXPRLamide residues that characterize pyrokinins, did not alter the activity of the cardiac neuromuscular system. However, a pyrokinin from the shrimp Litopenaeus vannamei [ADFAFNPRLamide, also known as Penaeus vannamei pyrokinin 2 (PevPK2)] increased both the frequency and amplitude of heart contractions when perfused through the isolated whole heart. None of the other crustacean pyrokinins tested (another from L. vannamei and two from the crab Cancer borealis) had any effect on the lobster heart. Similarly, altering the PevPK2 sequence either by truncation or by the substitution of single amino acids resulted in much lower or no activity in all cases; only the conservative substitution of serine for alanine at position 1 resulted in any activity on the heart. Thus, in contrast to other systems (cockroach and crab) in which all tested pyrokinins elicit similar bioactivities, activation of the pyrokinin receptor in the lobster heart appears to be highly isoform specific.
Neuropeptidergic signaling in the American Lobster Homarus Americanus: New insights from high-throughput nucleotide sequencing
Date: 2015-12-01
Creator: Andrew E. Christie, Megan Chi, Tess J. Lameyer, Micah G. Pascual, Devlin N., Shea, Meredith E. Stanhope, David J. Schulz, Patsy S. Dickinson
Access: Open access
- Peptides are the largest and most diverse class of molecules used for neurochemical communication, playing key roles in the control of essentially all aspects of physiology and behavior. The American lobster, Homarus americanus, is a crustacean of commercial and biomedical importance; lobster growth and reproduction are under neuropeptidergic control, and portions of the lobster nervous system serve as models for understanding the general principles underlying rhythmic motor behavior (including peptidergic neuromodulation). While a number of neuropeptides have been identified from H. americanus, and the effects of some have been investigated at the cellular/systems levels, little is currently known about the molecular components of neuropeptidergic signaling in the lobster. Here, a H. americanus neural transcriptome was generated and mined for sequences encoding putative peptide precursors and receptors; 35 precursor- and 41 receptor-encoding transcripts were identified. We predicted 194 distinct neuropeptides from the deduced precursor proteins, including members of the adipokinetic hormone-corazonin-like peptide, allatostatin A, allatostatin C, bursicon, CCHamide, corazonin, crustacean cardioactive peptide, crustacean hyperglycemic hormone (CHH), CHH precursor-related peptide, diuretic hormone 31, diuretic hormone 44, eclosion hormone, FLRFamide, GSEFLamide, insulin-like peptide, intocin, leucokinin, myosuppressin, neuroparsin, neuropeptide F, orcokinin, pigment dispersing hormone, proctolin, pyrokinin, SIFamide, sulfakinin and tachykinin-related peptide families. While some of the predicted peptides are known H. americanus isoforms, most are novel identifications, more than doubling the extant lobster neuropeptidome. The deduced receptor proteins are the first descriptions of H. americanus neuropeptide receptors, and include ones for most of the peptide groups mentioned earlier, as well as those for ecdysistriggering hormone, red pigment concentrating hormone and short neuropeptide F. Multiple receptors were identified for most peptide families. These data represent the most complete description of the molecular underpinnings of peptidergic signaling in H. americanus, and will serve as a foundation for future gene-based studies of neuropeptidergic control in the lobster.
Investigating the effects of a glutamine-rich protein on the localization of a mutant RNA-binding protein and stress response in Candida albicans Access to this record is restricted to members of the Bowdoin community. Log in here to view.
- Restriction End Date: 2028-06-01
Date: 2023-01-01
Creator: Christoph Anders Tatgenhorst
Access: Access restricted to the Bowdoin Community
The identification and visualization of candidate early embryonic patterning genes in Bradysia coprophila This record is embargoed.
- Embargo End Date: 2027-05-16
Date: 2024-01-01
Creator: Sarah Conant
Access: Embargoed
Demography of a Collapsing Aerial Insectivore Population
Date: 2017-05-01
Creator: Liam Taylor
Access: Open access
- Aerial insectivores have been declining across northeastern North America since the end of the 20th century. The mechanisms and demographic patterns of this decline are unclear. On Kent Island, New Brunswick, Canada, an isolated population of Tree Swallows (Tachycineta bicolor) collapsed between 1987 and 2010. To explore how demographic rates (i.e., survival, reproduction, and immigration) drove the population dynamics of these northeastern aerial insectivores, we combined productivity, population survey, and capture-recapture data in an integrated population model analysis. Neither consistently low juvenile survival rates, adult survival rates, nor clutch size were correlated with population growth rate across years. Alternatively, male and female immigration, hatching success, and fledging success rates were correlated with population growth rate. Because local hatching and fledging success rates cannot influence a population without local recruitment, we argue that the demography of these Tree Swallows is mainly structured by immigration. Parameter-substitution simulations revealed that overall decline was likely even if the population had avoided the worst years of demographic collapse. Breeding Bird Survey comparisons demonstrated how the Kent Island population represents both a demographic and geographical extreme at the edge of a declining region. These demographic patterns highlight the sensitivity, even to the point of local extinction, of some isolated populations to region-scale patterns in the production of potential immigrants.