Showing 1381 - 1390 of 2275 Items
Atmospheric measurements of the terrestrial O2 : CO2 exchange ratio of a midlatitude forest
Date: 2019-07-10
Creator: Mark O. Battle
J. William Munger
Margaret Conley
Eric Sofen
Rebecca, Perry
Ryan Hart
Zane Davis
Jacob Scheckman
Jayme Woogerd
Karina Graeter
Samuel Seekins
Sasha David
John Carpenter
Access: Open access
- Measurements of atmospheric O2 have been used to quantify large-scale fluxes of carbon between the oceans, atmosphere and land since 1992 (Keeling and Shertz, 1992). With time, datasets have grown and estimates of fluxes have become more precise, but a key uncertainty in these calculations is the exchange ratio of O2 and CO2 associated with the net land carbon sink (B). We present measurements of atmospheric O2 and CO2 collected over a 6-year period from a mixed deciduous forest in central Massachusetts, USA (42.537 N, 72.171 W). Using a differential fuel-cellbased instrument for O2 and a nondispersive infrared analyzer for CO2, we analyzed airstreams collected within and 5m above the forest canopy. Averaged over the entire period of record, we find these two species covary with a slope of -1:081±0:007 mol of O2 per mole of CO2 (the mean and standard error of 6 h periods). If we limit the data to values collected on summer days within the canopy, the slope is -1:03±0:01. These are the conditions in which biotic influences are most likely to dominate. This result is significantly different from the value of -1.1 widely used in O2-based calculations of the global carbon budget, suggesting the need for a deeper understanding of the exchange ratios of the various fluxes and pools comprising the net sink.
Thermal fractionation of air in polar firn by seasonal temperature gradients
Date: 2001-07-01
Creator: Jeffrey P. Severinghaus
Alexi Grachev
Mark Battle
Access: Open access
- Air withdrawn from the top 5-15 m of the polar snowpack (firn) shows anomalous enrichment of heavy gases during summer, including inert gases. Following earlier work, we ascribe this to thermal diffusion, the tendency of a gas mixture to separate in a temperature gradient, with heavier molecules migrating toward colder regions. Summer warmth creates a temperature gradient in the top few meters of the firn due to the thermal inertia of the underlying firn and causes gas fractionation by thermal diffusion. Here we explore and quantify this process further in order to (1) correct for bias caused by thermal diffusion in firn air and ice core air isotope records, (2) help calibrate a new technique for measuring temperature change in ice core gas records based on thermal diffusion [Severinghaus et al., 1998], and (3) address whether air in polar snow convects during winter and, if so, whether it creates a rectification of seasonality that could bias the ice core record. We sampled air at 2-m-depth intervals from the top 15 m of the firn at two Antarctic sites, Siple Dome and South Pole, including a winter sampling at the pole. We analyzed 15N/14N, 40Ar/36Ar, 40Ar/38Ar, 18O/16O of O2, O2/N2, 84Kr/36Ar, and 132Xe/36Ar. The results show the expected pattern of fractionation and match a gas diffusion model based on first principles to within 30%. Although absolute values of thermal diffusion sensitivities cannot be determined from the data with precision, relative values of different gas pairs may. At Siple Dome, δ40Ar/4 is 66 ± 2% as sensitive to thermal diffusion as δ15N, in agreement with laboratory calibration; δ18O/2 is 83 ± 3%, and δ84Kr/48 is 33 ± 3% as sensitive as δ15N. The corresponding figures for summer South Pole are 64 ± 2%, 81 ± 3%, and 34 ± 3%. Accounting for atmospheric change, the figure for δO2/N2/4 is 90 ± 3% at Siple Dome. Winter South Pole shows a strong depletion of heavy gases as expected. However, the data do not fit the model well in the deeper part of the profile and yield a systematic drift with depth in relative thermal diffusion sensitivities (except for Kr, constant at 34 ± 4%), suggesting the action of some other process that is not currently understood. No evidence for wintertime convection or a rectifier effect is seen.
Functions of the ectodomain and cytoplasmic tyrosine phosphatase domains of receptor protein tyrosine phosphatase Dlar in vivo
Date: 2003-10-01
Creator: Neil X. Krueger
R. Sreekantha Reddy
Karl Johnson
Jack Bateman
Nancy, Kaufmann
Daniella Scalice
David Van Vactor
Haruo Saito
Access: Open access
- The receptor protein tyrosine phosphatase (PTPase) Dlar has an ectodomain consisting of three immunoglobulin (Ig)-like domains and nine fibronectin type III (FnIII) repeats and a cytoplasmic domain consisting of two PTPase domains, membrane-proximal PTP-D1 and C-terminal PTP-D2. A series of mutant Dlar transgenes were introduced into the Drosophila genome via P-element transformation and were then assayed for their capacity to rescue phenotypes caused by homozygous loss-of-function genotypes. The Ig-like domains, but not the FnIII domains, are essential for survival. Conversely, the FnIII domains, but not the Ig-like domains, are required during oogenesis, suggesting that different domains of the Dlar ectodomain are involved in distinct functions during Drosophila development. All detectable PTPase activity maps to PTP-D1 in vitro. The catalytically inactive mutants of Dlar were able to rescue Dlar -/- lethality nearly as efficiently as wild-type Dlar transgenes, while this ability was impaired in the PTP-D2 deletion mutants DlarΔPTP-D2 and Dlarbypass. Dlar-C1929S, in which PTP-D2 has been inactivated, increases the frequency of bypass phenotype observed in Dlar-/- genotypes, but only if PTP-D1 is catalytically active in the transgene. These results indicate multiple roles for PTP-D2, perhaps by acting as a docking domain for downstream elements and as a regulator of PTP-D1.
The receptor tyrosine phosphatase Dlar and integrins organize actin filaments in the Drosophila follicular epithelium
Date: 2001-09-04
Creator: Jack Bateman
R. Srekantha Reddy
Haruo Saito
David Van Vactor
Access: Open access
- Background: Regulation of actin structures is instrumental in maintaining proper cytoarchitecture in many tissues. In the follicular epithelium of Drosophila ovaries, a system of actin filaments is coordinated across the basal surface of cells encircling the oocyte. These filaments have been postulated to regulate oocyte elongation; however, the molecular components that control this cytoskeletal array are not yet understood. Results: We find that the receptor tyrosine phosphatase (RPTP) Dlar and integrins are involved in organizing basal actin filaments in follicle cells. Mutations in Dlar and the common β-integrin subunit mys cause a failure in oocyte elongation, which is correlated with a loss of proper actin filament organization. Immunolocalization shows that early in oogenesis Dlar is polarized to membranes where filaments terminate but becomes generally distributed late in development, at which time β-integrin and Enabled specifically associate with actin filament terminals. Rescue experiments point to the early period of polar Dlar localization as critical for its function. Furthermore, clonal analysis shows that loss of Dlar or mys influences actin filament polarity in wild-type cells that surround mutant tissues, suggesting that communication between neighboring cells regulates cytoskeletal organization. Finally, we find that two integrin α subunits encoded by mew and if are required for proper oocyte elongation, implying that multiple components of the ECM are instructive in coordinating actin fiber polarity. Conclusions: Dlar cooperates with integrins to coordinate actin filaments at the basal surface of the follicular epithelium. To our knowledge, this is the first direct demonstration of an RPTP's influence on the actin cytoskeleton.
The guanine nucleotide exchange factor trio mediates axonal development in the Drosophila embryo
Date: 2000-01-01
Creator: Jack Bateman
Huidy Shu
David Van Vactor
Access: Open access
- Recent analysis of Rho subfamily GTPases in Drosophila revealed roles for Rac and Cdc42 during axonogenesis. Here, we describe the identification and characterization of the Drosophila counterpart of Trio, a guanine nucleotide exchange factor (GEF) that associates with the receptor phosphatase LAR and regulates GTPase activation in vertebrate cells. Mutants deficient in trio activity display defects in both central and peripheral axon pathways reminiscent of pheno-types observed in embryos deficient in small GTPase function. Double mutant analysis shows that trio interacts with Rac in a dose-sensitive manner but not with Rho. Moreover, reduction of trio activity potentiates the phenotype of mutations in the LAR homolog Dlar, suggesting that these proteins collaborate in orchestrating the cytoskeletal events that underlie normal axonogenesis.
Inclusive decay B→ηX
Date: 1996-01-01
Creator: Y. Kubota
M. Lattery
M. Momayezi
J. K. Nelson
S., Patton
R. Poling
V. Savinov
S. Schrenk
R. Wang
M. S. Alam
I. J. Kim
Z. Ling
A. H. Mahmood
J. J. O’Neill
H. Severini
C. R. Sun
F. Wappler
G. Crawford
C. M. Daubenmier
R. Fulton
D. Fujino
K. K. Gan
K. Honscheid
H. Kagan
R. Kass
J. Lee
M. Sung
C. White
A. Wolf
M. M. Zoeller
F. Butler
Access: Open access
- Using data samples taken at the Υ(4S) resonance and nearby continuum e+e- annihilation with the CLEO-II detector at CESR, we have measured the inclusive branching fraction B(B→ηX)=(17.6±1.1±1.2)%, and the momentum distribution of the η mesons from B meson decay. The η yield cannot be explained as arising solely from the decay of intermediate charmed mesons. © 1996 The American Physical Society.
Measurement of the decay asymmetry parameters in Λc+ → Λπ+ and Λc+ → Σ+π0
Date: 1995-05-11
Creator: M. Bishai
J. Fast
E. Gerndt
J. W. Hinson
R. L., McIlwain
T. Miao
D. H. Miller
M. Modesitt
D. Payne
E. I. Shibata
I. P.J. Shipsey
P. N. Wang
M. Battle
J. Ernst
L. Gibbons
Y. Kwon
S. Roberts
E. H. Thorndike
C. H. Wang
J. Dominick
M. Lambrecht
S. Sanghera
V. Shelkov
T. Skwarnicki
R. Stroynowski
I. Volobouev
G. Wei
M. Artuso
M. Gao
M. Goldberg
D. He
Access: Open access
- We have measured the weak decay asymmetry parameters (αΛc) for two Λc+ decay modes. Our measurements are αΛc = -0.94-0.06-0.06+0.21+0.12 for the decay mode Λc+ → Λπ+ and αΛc = -0.45 ±0.31 ±0.06 for the decay mode Λc → Σ+ π0. By combining these measurements with the previously measured decay rates, we have extracted the parity-violating and parity-conserving amplitudes. These amplitudes are used to test models of nonleptonic charmed baryon decay. © 1995.
Targeted identification of glycosylated proteins in the gastric pathogen helicobacter pylori (Hp)
Date: 2013-09-01
Creator: Kanokwan Champasa
Scott A. Longwell
Aimee M. Eldridge
Elizabeth A. Stemmler
Danielle H., Dube
Access: Open access
- Virulence of the gastric pathogen Helicobacter pylori (Hp) is directly linked to the pathogen's ability to glycosylate proteins; for example, Hp flagellin proteins are heavily glycosylated with the unusual nine-carbon sugar pseudaminic acid, and this modification is absolutely essential for Hp to synthesize functional flagella and colonize the host's stomach. Although Hp's glycans are linked to pathogenesis, Hp's glycome remains poorly understood; only the two flagellin glycoproteins have been firmly characterized in Hp. Evidence from our laboratory suggests that Hp synthesizes a large number of as-yet unidentified glycoproteins. Here we set out to discover Hp's glycoproteins by coupling glycan metabolic labeling with mass spectrometry analysis. An assessment of the subcellular distribution of azide-labeled proteins by Western blot analysis indicated that glycoproteins are present throughout Hp and may therefore serve diverse functions. To identify these species, Hp's azide-labeled glycoproteins were tagged via Staudinger ligation, enriched by tandem affinity chromatography, and analyzed by multidimensional protein identification technology. Direct comparison of enriched azide-labeled glycoproteins with a mock-enriched control by both SDS-PAGE and mass spectrometry-based analyses confirmed the selective enrichment of azide-labeled glycoproteins. We identified 125 candidate glycoproteins with diverse biological functions, including those linked with pathogenesis. Mass spectrometry analyses of enriched azide-labeled glycoproteins before and after cleavage of O-linked glycans revealed the presence of Staudinger ligation-glycan adducts in samples only after beta-elimination, confirming the synthesis of O-linked glycoproteins in Hp. Finally, the secreted colonization factors urease alpha and urease beta were biochemically validated as glycosylated proteins via Western blot analysis as well as by mass spectrometry analysis of cleaved glycan products. These data set the stage for the development of glycosylation-based therapeutic strategies, such as new vaccines based on natively glycosylated Hp proteins, to eradicate Hp infection. Broadly, this report validates metabolic labeling as an effective and efficient approach for the identification of bacterial glycoproteins. © 2013 by The American Society for Biochemistry and Molecular Biology, Inc.
Luminosity measurement with the CLEO II detector
Date: 1994-07-01
Creator: G. Crawford
C. M. Daubenmier
R. Fulton
D. Fujino
K. K., Gan
K. Honscheid
H. Kagan
R. Kass
J. Lee
R. Malchow
Y. Skovpen
M. Sung
C. White
F. Butler
X. Fu
G. Kalbfleisch
W. R. Ross
P. Skubic
M. Wood
J. Fast
R. L. McIlwain
T. Miao
D. H. Miller
M. Modesitt
D. Payne
E. I. Shibata
I. P.J. Shipsey
P. N. Wang
M. Battle
J. Ernst
L. Gibbons
Access: Open access
- A measurement of absolute integrated luminosity is presented using the CLEO II detector operating at the CESR e+e- storage ring. Independent analyses of three different final states (e+e-, γγ, and μ+μ-) at √s {reversed tilde equals} 10 GeV normalize to the expected theoretical cross sections and correct for detection efficiencies. The resulting luminosities are measured with systematic errors of ±1.8%, ±1.6%, and ±2.2%, respectively, and are consistent with one another. The combined luminosity has a systematic error of ±1.0%. © 1994.
Study of the decay Λc+→λl+νl
Date: 1994-03-10
Creator: T. Bergfeld
B. I. Eisenstein
G. Gollin
B. Ong
M., Palmer
M. Selen
J. J. Thaler
A. J. Sadoff
R. Ammar
S. Ball
P. Baringer
A. Bean
D. Besson
D. Coppage
N. Copty
R. Davis
N. Hancock
M. Kelly
N. Kwak
H. Lam
Y. Kubota
M. Lattery
J. K. Nelson
D. Patton
D. Perticone
R. Poling
V. Savinov
S. Schrenk
R. Wang
M. S. Alam
I. J. Kim
Access: Open access
- Using the CLEO II detector at CESR we observe 500 Λl+ pairs consistent with the semileptonic decay Λc+ → λ+ν We measure σ(e+e- → Λ+cX) · B(Λ+c → Λl+νl) = 4.77±0.25±0.66 pb. Combining with the charm semileptonic width and the lifetime of the Λc we also obtain B(Λ+c → pK-π+). We find no evidence for Λl+νl final states in which there are additional Λ+c decay products. We measure the decay asymmetry parameter of Λ+c → Λe+νe to be αΛc = -0.89+0.17+0.09-0.11-0.05. © 1994.