Showing 1371 - 1380 of 5240 Items
Peripheral modulation of cardiac contractions in the American lobster, Homarus americanus, by the peptide myosuppressin is mediated by effects on the cardiac muscle itself
Date: 2023-01-01
Creator: Isabel Stella Petropoulos
Access: Open access
- A substantial factor for behavioral flexibility is modulation — largely via neuropeptides — which occurs at multiple sites including neurons, muscles, and the neuromuscular junction (NMJ). Complex modulation distributed across multiple sites provides an interesting question: does modulation at multiple locations lead to greater dynamics than one receptor site alone? The cardiac neuromuscular system of the American lobster (Homarus americanus), driven by a central pattern generator called the cardiac ganglion (CG), is a model system for peptide modulation. The peptide myosuppressin (pQDLDHVFLRFamide) has been shown in the whole heart to decrease contraction frequency, largely due to its effects on the CG, as well as increase contraction amplitude by acting on periphery of the neuromuscular system, either at the cardiac muscle, the NMJ, or both. This set of experiments addresses the location(s) at which myosuppressin exerts its effects at the periphery. To elucidate myosuppressin’s effects on the cardiac muscle, the CG was removed, and muscle contractions were stimulated with L-glutamate while superfusing myosuppressin. Myosuppressin increased glutamate-evoked contraction amplitude in the isolated muscle, suggesting that myosuppressin exerts its peripheral effects directly on the cardiac muscle. To examine effects on the NMJ, excitatory junction potentials were evoked by stimulating of the motor nerve and intracellularly recording a single muscle fiber both in control saline and in the presence of myosuppressin. Myosuppressin did not modulate the amplitude of EJPs suggesting myosuppressin acts at the muscle and not at the NMJ, to cause an increase in contraction amplitude.
Host and symbiont-specific patterns of gene expression in response to cold stress in the temperate coral Astrangia poculata
Date: 2023-01-01
Creator: Kellie Navarro
Access: Open access
- The coral Astrangia poculata inhabits hard-bottom environments from the Gulf of Mexico to Massachusetts and withstands large seasonal variation in temperature (–2 to 26 °C). This thermal range and its ability to live in a facultative symbiosis makes this species an ideal model system for investigating stress responses to ocean temperature variation. Although it has been shown that aposymbiotic A. poculata upregulates more genes in response to cold stress than heat stress, the transcriptomic response of the holobiont (coral host and symbiotic algae) to stress is unknown. In this study, we characterize changes in gene expression in both the host and symbionts under cold stress (6ºC) and ambient (12ºC) seawater temperatures. We use RNAseq to visualize how patterns of global gene expression change in response to these temperatures within the transcriptomes of replicate corals (n=10, each temperature) and their symbiont partners. By filtering the holobiont assembly for known coral host and symbiont genes, we contrasted patterns of differential expression (DE) for each partner and the functional processes for each set of DE genes. Differential gene expression analyses revealed that the cnidarian coral host responds strongly to cold stress, while algal symbionts did not have a significant stress response. In the coral host, we found up-regulation of biological processes associated with DNA repair, immunity, and maintaining cellular homeostasis as well as downregulation of mechanisms associated with DNA repair and RNA splicing, indicating inhibition of necessary cellular processes due to environmental stress.
Twentieth Century American Watercolors
Date: 1973-01-01
Access: Open access
- Exhibition catalogue from Bowdoin College Museum of Art, November 2-December 9, 1973.
General Catalogue of Bowdoin College and the Medical School of Maine: A Biographical Record of Alumni and Officers, 1794-1950
Date: 1950-01-01
Access: Open access
- General Catalogue of Bowdoin College and the Medical School of Maine: A Biographical Record of Alumni and Officers, 1794-1950 (1950) provides a complete and comprehensive biographical record of all of Bowdoin’s students, faculty, and administrative officers from the founding of the College in 1794 through 1950.
Reflections questionnaire response by Anonymous on March 22, 2021
Date: 2021-01-01
Creator: Anonymous
Access: Open access
- This is a response to the Documenting Bowdoin & COVID-19 Reflections Questionnaire. The questionnaire was created in March 2021 by staff of Bowdoin's George J. Mitchell Department of Special Collections & Archives. The author is a member of Bowdoin's staff.